LRP-1 Cluster III Antibody [Unconjugated]

LRP-1 Cluster III was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human LRP-1 Cluster III Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRP-1 Cluster III Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived human LRP-1 Cluster III
  Ser2522-Ile2941
  Accession # Q07954
• Specificity: Detects human LRP-1 Cluster III in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-1 Cluster III Antibody [Unconjugated]

• A2MR;APOER;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;LRP;LRP1A;Prolow-density lipoprotein receptor-related protein 1;TGFBR5
• LRP1 Cluster III
• LRP-1 Cluster III

Background

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta , NMDA receptor subunits, TGF-beta receptors, Frizzled‑1, various integrins, and the prion protein PrP^C (1, 5‑10). Human LRP-1 is an N‑glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane  beta chain and a 515 kDa  alpha chain that associates noncovalently with the  beta chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF‑like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). LRP-1 Cluster III (aa 2522‑2941) contains ten LDLR-A cysteine-rich domains (14). Within this region, human LRP-1 shares 97% aa sequence identity with mouse and rat LRP-1. A soluble form of LRP-1 is shed into the serum and cerebrospinal fluid and retains ligand binding properties (15, 16). LRP-1 Cluster III contains binding sites for LRPAP/RAP (14).

1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
2. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
5. Muratoglu, S.C. et al. (2010) J. Biol. Chem. 258:14308.
6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
11. Herz, J. et al. (1990) EMBO J. 9:1769.
12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
13. Herz, J. et al. (1988) EMBO J. 7:4119.
14. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
15. Liu, Q. et al. (2009) Mol. Neurodegener. 4:17.
16. Gorovoy, M. et al. (2010) J. Leukoc. Biol. Jul 7 epub.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRP1
• Uniprot:
  • Q07954(Human)