LRP-1 Cluster III Antibody [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 555

LRP-1 Cluster III Antibody [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human LRP-1 Cluster III in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-1 Cluster III Antibody [Alexa Fluor™ Plus 555]

• A2MR;APOER;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;LRP;LRP1A;Prolow-density lipoprotein receptor-related protein 1;TGFBR5
• LRP1 Cluster III
• LRP-1 Cluster III

Background

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta , NMDA receptor subunits, TGF-beta receptors, Frizzled‑1, various integrins, and the prion protein PrP^C (1, 5‑10). Human LRP-1 is an N‑glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane  beta chain and a 515 kDa  alpha chain that associates noncovalently with the  beta chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF‑like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). LRP-1 Cluster III (aa 2522‑2941) contains ten LDLR-A cysteine-rich domains (14). Within this region, human LRP-1 shares 97% aa sequence identity with mouse and rat LRP-1. A soluble form of LRP-1 is shed into the serum and cerebrospinal fluid and retains ligand binding properties (15, 16). LRP-1 Cluster III contains binding sites for LRPAP/RAP (14).

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2. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
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4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
5. Muratoglu, S.C. et al. (2010) J. Biol. Chem. 258:14308.
6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
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8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
11. Herz, J. et al. (1990) EMBO J. 9:1769.
12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
13. Herz, J. et al. (1988) EMBO J. 7:4119.
14. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
15. Liu, Q. et al. (2009) Mol. Neurodegener. 4:17.
16. Gorovoy, M. et al. (2010) J. Leukoc. Biol. Jul 7 epub.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.