LRP-1 Cluster II Antibody [Unconjugated]

LRP-1 Cluster II was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human LRP-1 Cluster II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2368) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LRP‑1 Cluster II was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human LRP‑1 Cluster II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2368) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LRP-1 Cluster II Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LRP‑1 Cluster II
  Arg786-Leu1165
  Accession # Q07954
• Specificity: Detects human LRP‑1 Cluster II in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LRP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-1 Cluster II Antibody [Unconjugated]

• A2MR
• A2MR;APOER;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;LRP;LRP1A;Prolow-density lipoprotein receptor-related protein 1;TGFBR5
• Alpha-2-Macroglobulin Receptor
• APOER
• APR
• CD91
• LRP1 Cluster II
• LRP-1 Cluster II
• LRP1
• TGFBR5

Background

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2‑macroglobulin receptor, is a type I transmembrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and the inhibition of serine proteases (1‑4). LRP-1 also associates, or through intracellular scaffold proteins, with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta , NMDA receptor subunits, TGF-beta receptors, Frizzled-1, various integrins, and the prion protein PrP^C (1, 5‑10). Human LRP‑1 is N glycosylated and sialylated, and cleaved in the Golgi to produce an 85 kDa transmembrane beta chain, and a 515 kDa alpha chain. Both associate noncovalently, with the beta chain remaining completely extracellular (11, 12). The alpha chain of LRP 1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF-like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). Cluster II (aa 786 - 1165) contains one EGF-like and eight LDLR class A repeats (14, 15). Cluster II contains binding sites for Apolipoprotein E, LPL, LRPAP/RAP, alpha 2 Macroglobulin, Coagulation Factor VIII light chain, Lactoferrin, PAI-1, tPA-PAI-1 complexes, Pro‑uPA, and TFPI (14, 15). Within this region, human LRP-1 shares 99% aa sequence identity with mouse and rat LRP-1. A shed soluble form of LRP-1 circulates in the serum and retains ligand binding properties (16).

1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
2. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
5. Takayama, Y. et al. (2005) J. Biol. Chem. 280:18504.
6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
11. Herz, J. et al. (1990) EMBO J. 9:1769.
12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
13. Herz, J. et al. (1988) EMBO J. 7:4119.
14. Horn, I.R. et al. (1997) J. Biol. Chem. 272:13608.
15. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
16. Quinn, K.A. et al. (1999) Exp. Cell Res. 251:433.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRP-1 Cluster II Antibody (AF2368)(4)

Publications using AF2368

• Mariko Yamada, Hideki Hayashi, Kaori Suzuki, Shoko Sato, Daisuke Inoue, Yui Iwatani et al. Furin-mediated cleavage of LRP1 and increase in ICD of LRP1 after cerebral ischemia and after exposure of cultured neurons to NMDA Scientific Reports 8/13/2019 [PMID: 31409872]
• Mariko Yamada, Hideki Hayashi, Kaori Suzuki, Shoko Sato, Daisuke Inoue, Yui Iwatani et al. Furin-mediated cleavage of LRP1 and increase in ICD of LRP1 after cerebral ischemia and after exposure of cultured neurons to NMDA Scientific Reports 2019-08-13 [PMID: 31409872] (Western Blot, Immunocytochemistry, Rat)
• S Toldo, D Austin, AG Mauro, E Mezzaroma, BW Van Tassel, C Marchetti, S Carbone, S Mogelsvang, C Gelber, A Abbate Low-Density Lipoprotein Receptor-Related Protein-1 Is a Therapeutic Target in Acute�Myocardial Infarction JACC Basic Transl Sci, 2017-10-30;2(5):561-574. 2017-10-30 [PMID: 30062170] (Western Blot)
• Chien YJ, Chen WJ, Hsu WL, Chiou SS Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein. Virology, 2008-07-21;379(1):143-51. 2008-07-21 [PMID: 18640695] (Neutralization, Hamster)

Bioinformatics

• Gene Symbol: LRP1
• Uniprot:
  • Q07954(Human)