LRP-1 Cluster II Antibody [Unconjugated]

Images

 
LRP‑1 Cluster II was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human LRP‑1 Cluster II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2368) at 3 ...read more
LRP‑1 Cluster II was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human LRP‑1 Cluster II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2368) at 3 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

LRP-1 Cluster II Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRP‑1 Cluster II
Arg786-Leu1165
Accession # Q07954
Specificity
Detects human LRP‑1 Cluster II in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) CRP-6 and rhMegalin-C4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LRP1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Immunocytochemistry 3-15 ug/mL
Publications
Read Publications using
AF2368 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-1 Cluster II Antibody [Unconjugated]

  • A2MR
  • Alpha-2-Macroglobulin Receptor
  • APOER
  • APR
  • CD91
  • LRP1 Cluster II
  • LRP-1 Cluster II
  • LRP1
  • TGFBR5

Background

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2‑macroglobulin receptor, is a type I transmembrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and the inhibition of serine proteases (1‑4). LRP-1 also associates, or through intracellular scaffold proteins, with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled-1, various integrins, and the prion protein PrPC (1, 5‑10). Human LRP‑1 is N glycosylated and sialylated, and cleaved in the Golgi to produce an 85 kDa transmembrane beta chain, and a 515 kDa alpha chain. Both associate noncovalently, with the beta chain remaining completely extracellular (11, 12). The alpha chain of LRP 1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF-like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). Cluster II (aa 786 - 1165) contains one EGF-like and eight LDLR class A repeats (14, 15). Cluster II contains binding sites for Apolipoprotein E, LPL, LRPAP/RAP, alpha 2 Macroglobulin, Coagulation Factor VIII light chain, Lactoferrin, PAI-1, tPA-PAI-1 complexes, Pro‑uPA, and TFPI (14, 15). Within this region, human LRP-1 shares 99% aa sequence identity with mouse and rat LRP-1. A shed soluble form of LRP-1 circulates in the serum and retains ligand binding properties (16).

  1. Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
  2. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
  3. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
  4. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
  5. Takayama, Y. et al. (2005) J. Biol. Chem. 280:18504.
  6. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
  7. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  8. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  9. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
  10. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
  11. Herz, J. et al. (1990) EMBO J. 9:1769.
  12. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
  13. Herz, J. et al. (1988) EMBO J. 7:4119.
  14. Horn, I.R. et al. (1997) J. Biol. Chem. 272:13608.
  15. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
  16. Quinn, K.A. et al. (1999) Exp. Cell Res. 251:433.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82820
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB400-156
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready, Dual ISH-IHC
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA
NBP2-67667
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-89334
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13075
Species: Hu, Mu, Rt, Pm
Applications: WB, DB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP2-57299
Species: Hu
Applications: WB, IHC, IHC-P
H00065108-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
NBP2-67279
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-13415
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-45041
Species: Hu, Po
Applications: Flow, ICC/IF, IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP2-19417
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF2368
Species: Hu
Applications: WB, IHC, ICC/IF

Publications for LRP-1 Cluster II Antibody (AF2368)(2)

We have publications tested in 1 confirmed species: Hamster.

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Hamster
(1)
All Species

Reviews for LRP-1 Cluster II Antibody (AF2368) (0)

There are no reviews for LRP-1 Cluster II Antibody (AF2368). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LRP-1 Cluster II Antibody (AF2368) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional LRP-1 Cluster II Products

Bioinformatics Tool for LRP-1 Cluster II Antibody (AF2368)

Discover related pathways, diseases and genes to LRP-1 Cluster II Antibody (AF2368). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LRP-1 Cluster II Antibody (AF2368)

Discover more about diseases related to LRP-1 Cluster II Antibody (AF2368).
 

Pathways for LRP-1 Cluster II Antibody (AF2368)

View related products by pathway.

PTMs for LRP-1 Cluster II Antibody (AF2368)

Learn more about PTMs related to LRP-1 Cluster II Antibody (AF2368).

Blogs on LRP-1 Cluster II

There are no specific blogs for LRP-1 Cluster II, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LRP-1 Cluster II Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol LRP1
Uniprot