LNK/SH2B3 Antibody

Western Blot: LNK/SH2B3 Antibody [NB100-2295] - NB100-2295 (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: LNK/SH2B3 Antibody [NB100-2295] - HEK293 overexpressing SH2B3 and probed with (mock transfection in first lane).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LNK/SH2B3 Antibody Summary

• Description: Novus Biologicals Goat LNK/SH2B3 Antibody (NB100-2295) is a polyclonal antibody validated for use in WB and ELISA. Anti-LNK/SH2B3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence NGPALQPSSPC corresponding to N-Terminus according to NP_005466.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SH2B3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1.0 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in MOLT4 lysates (calculated MW of 63.2 kDa band according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  LNK/SH2B3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LNK/SH2B3 Antibody

• IDDM20
• LNK
• LNKSH2B adapter protein 3
• Lymphocyte adapter protein
• Lymphocyte-specific adapter protein Lnk
• SH2B adaptor protein 3
• SH2B3
• Signal transduction protein Lnk

Background

T-cell activation requires stimulation of the T-cell receptor (TCR; see MIM 186880)-CD3 (see CD3Z; MIM 186780) complex, followed by recruitment of an array of intracellular signaling proteins (e.g., GRB2 (MIM 108355) and PLCG1 (MIM 172420)). Mediating the interaction between the extracellular receptors and intracellular signaling pathways are adaptor proteins such as LAT (MIM 602354), TRIM (MIM 604962), and LNK.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LNK/SH2B3 Antibody (NB100-2295)(2)

Publications using NB100-2295

• Gery S, Cao Q, Gueller S et al. Lnk inhibits myeloproliferative disorder-associated JAK2 mutant, JAK2V617F. J Leukoc Biol 2009:jlb0908575. [PMID: 19293402]
• Li Y, He X, Schembri-King J et al. Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation. J Immunol 2000-05-15 [PMID: 10799879]

Bioinformatics

• Gene Symbol: SH2B3
• Entrez:
  • 10019(Human)
• Uniprot:
  • NP_005466.(Human)