LNK/SH2B3 Overexpression Lysate (Native)

Images

 
Western Blot: LNK Overexpression Lysate (Adult Normal) [NBL1-15917] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LNK.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LNK/SH2B3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC218359 and based on accession number NM_005475. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SH2B adaptor protein 3 (SH2B3), mRNA.
Gene
SH2B3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for LNK/SH2B3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LNK/SH2B3 Overexpression Lysate (Native)

  • IDDM20
  • LNK
  • LNKSH2B adapter protein 3
  • Lymphocyte adapter protein
  • Lymphocyte-specific adapter protein Lnk
  • SH2B adaptor protein 3
  • SH2B3
  • Signal transduction protein Lnk

Background

T-cell activation requires stimulation of the T-cell receptor (TCR; see MIM 186880)-CD3 (see CD3Z; MIM 186780) complex, followed by recruitment of an array of intracellular signaling proteins (e.g., GRB2 (MIM 108355) and PLCG1 (MIM 172420)). Mediating the interaction between the extracellular receptors and intracellular signaling pathways are adaptor proteins such as LAT (MIM 602354), TRIM (MIM 604962), and LNK.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2988
Species: Mu, Rt
Applications: WB
NBP2-13305
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF6915
Species: Hu, Mu, Rt
Applications: WB
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
AF1016
Species: Hu
Applications: WB, Neut
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-02300
Species: Hu
Applications: WB, Flow, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-52084
Species: Hu, Mu, Rt
Applications: WB, IHC-P, PEP-ELISA
MAB3428
Species: Hu
Applications: WB, IHC
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready

Publications for LNK/SH2B3 Lysate (NBL1-15917) (0)

There are no publications for LNK/SH2B3 Lysate (NBL1-15917).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LNK/SH2B3 Lysate (NBL1-15917) (0)

There are no reviews for LNK/SH2B3 Lysate (NBL1-15917). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LNK/SH2B3 Lysate (NBL1-15917) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional LNK/SH2B3 Products

Bioinformatics Tool for LNK/SH2B3 Lysate (NBL1-15917)

Discover related pathways, diseases and genes to LNK/SH2B3 Lysate (NBL1-15917). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LNK/SH2B3 Lysate (NBL1-15917)

Discover more about diseases related to LNK/SH2B3 Lysate (NBL1-15917).
 

Pathways for LNK/SH2B3 Lysate (NBL1-15917)

View related products by pathway.

PTMs for LNK/SH2B3 Lysate (NBL1-15917)

Learn more about PTMs related to LNK/SH2B3 Lysate (NBL1-15917).

Blogs on LNK/SH2B3

There are no specific blogs for LNK/SH2B3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-2 [Unconjugated]
202-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LNK/SH2B3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SH2B3
Entrez