LMP7/PSMB8 Antibody

Western Blot: LMP7/PSMB8 Antibody [NB120-3329] - Analysis of HeLa cell extract.

• Reactivity: Hu, Mu, Rt, Sh
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

LMP7/PSMB8 Antibody Summary

• Immunogen: Synthetic Peptide: C V(259) E S T D V S D L L H Q Y R E A(274)
• Predicted Species: Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMB8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LMP7/PSMB8 Antibody

• ALDD
• beta5i
• D6S216E
• EC 3.4.25.1
• JMP
• LMP7
• LMP7MGC1491
• Low molecular mass protein 7
• low molecular weight protein 7
• Macropain subunit C13
• Multicatalytic endopeptidase complex subunit C13
• NKJO
• protease component C13
• proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7)
• proteasome catalytic subunit 3i
• Proteasome Component C13
• proteasome subunit beta 5i
• proteasome subunit beta type-8
• Proteasome subunit beta-5i
• Proteasome Subunit Y2
• proteasome-related gene 7
• PSMB5i
• PSMB5iD6S216
• PSMB8
• Really interesting new gene 10 protein
• RING10
• RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7)
• Y2

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.

The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LMP7/PSMB8 Antibody (NB120-3329)(2)

Publications using NB120-3329

• Lang VR, Mielenz D, Neubert K et al. The early marginal zone B cell-initiated T-independent type 2 response resists the proteasome inhibitor bortezomib. J Immunol 2010-11-01 [PMID: 20921528] (WB, Mouse)
• Meister S, Frey B, Lang VR et al. Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells. Neoplasia. 2010-07-01 [PMID: 20651984] (WB, Human)

Bioinformatics

• Gene Symbol: PSMB8
• Entrez:
  • 5696(Human)
• Uniprot:
  • P28062()