Human LL-37 ELISA Kit (Colorimetric)

ELISA: Human LL-37 ELISA Kit (Colorimetric) [NBP3-06932] - Samples were spiked with high concentrations of Human LL-37 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human LL-37 ELISA Kit (Colorimetric) [NBP3-06932] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Human LL-37 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.46
• Intra-Assay: 5.51
• Spike Recovery: 92-109 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CAMP

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human LL-37 ELISA Kit (Colorimetric)

• 18 kDa cationic antimicrobial protein
• Antibacterial peptide FALL-39
• CAP18
• CAP-18
• cathelicidin antimicrobial peptide
• CRAMP
• epididymis secretory sperm binding protein
• FALL-39 peptide antibiotic
• FALL39
• FALL-39
• hCAP-181
• HSD26
• LL37

Background

Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The active biologic domains of the cathelicidins generally reside in the C terminus. The C terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for LL-37 ELISA Kit (NBP3-06932)(2)

Publications using NBP3-06932

• Aman Damara, Joanna Wegner, Emily R Trzeciak, Antonia Kolb, Mahsa Nastaranpour, Rahul Khatri, Andrea Tuettenberg, Daniela Kramer, Stephan Grabbe, Fatemeh Shahneh LL37/self-DNA complexes mediate monocyte reprogramming. Clinical immunology (Orlando, Fla.) 2024-06-21 [PMID: 38909973]
• Lu P, Shan M, Ji X et al. EPURISp: Combining Enzymatic Digestion, Ultrafiltration, and Rapid In Situ Sample Purification for High-performance Proteomics Journal of proteome research 2023-09-25 [PMID: 37747260] (ELISA)

FAQs for LL-37 ELISA Kit (NBP3-06932). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CAMP