LKB1/STK11 Antibody [Unconjugated]

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LKB1/STK11 knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Sheep ...read more
LKB1/STK11 was detected in formalin fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8055) at 1 µg/mL overnight ...read more
LKB1/STK11 was detected in formalin fixed paraffin-embedded sections of human breast tissue using Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8055) at 10 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

LKB1/STK11 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human LKB1/STK11
Met1-Gln433
Accession # Q15831
Specificity
Detects human LKB1/STK11 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
STK11
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
Control
LKB1/STK11 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LKB1/STK11 Antibody [Unconjugated]

  • EC 2.7.11.1
  • LKB1 serine/threonine kinase 11 (Peutz-Jeghers syndrome)
  • LKB1
  • PJS polarization-related protein LKB1
  • PJS
  • Renal carcinoma antigen NY-REN-19
  • serine/threonine kinase 11
  • serine/threonine-protein kinase 11
  • Serine/threonine-protein kinase LKB1
  • STK11

Background

LKB1 (Liver kinase B1; also STK11/Ser/Thr-protein kinase 11 and NY-REN19) is a 55 kDa intracellular member of the LKB1 subfamily, CAMK Ser/Thr protein kinase family of molecules. It is widely expressed, being particularly investigated in small intestinal columnar epithelium and hepatocytes. STK11 is associated with a wide variety of functions, including the initiation of apoptosis through binding to p53, the regulation of TGF-beta signaling through the creation of a SMAD4-LIP1-STK11 complex, and the generation of an epithelial polarized phenotype via cytoskeletal remodeling. Schematically, it would appear that STK11 acts, at least in part, through its ability to phosphorylate multiple AMPK-related kinases, as well as AMPK itself. Human STK11 is 433 amino acids (aa) in length. It potentially contains a three aa propeptide at its C-terminus. The mature segment (aa 1-430) possesses one large protein kinase domain (aa 49-309) plus at least four utilized phosphorylation sites.  Phosphorylation on Ser428 promotes the ability of LKB1 to suppress G361 cell growth. Palmitoylation is suggested to occur on Cys418. There are three alternative splice variants. Either individually or in combination, they involve an insertion of nine aa after Tyr126 and a 34 aa substitution for aa 371-433. Full-length human STK11 shares 90% aa sequence identity with mouse STK11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol STK11
Uniprot