Lipin 3 Antibody

Immunohistochemistry-Paraffin: Lipin 3 Antibody [NB100-53802] - (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Lipin 3 Antibody Summary

• Description: Novus Biologicals Goat Lipin 3 Antibody (NB100-53802) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Lipin 3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KPKQKEDAVATD corresponding to internal region according to NP_075047.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LPIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave bands at approx. 80 kDa band and 60 kDa band in human liver lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6 kDa band according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). IHC-P: Human thyroid gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lipin 3 Antibody

• dJ450M14.2
• dJ450M14.3
• dJ620E11.2
• EC 3.1.3.4
• Lipin 3
• lipin 3-like
• lipin-3
• Lipin-3-like
• LIPN3L
• LPIN3
• phosphatidate phosphatase LPIN3
• SMP2

Background

Humans lipodystrophy is characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mice carrying mutations in the fatty liver dystrophy (fld) gene have similar phenotypes. Through positional cloning, the mouse gene responsible for fatty liver dystrophy was isolated and designated Lpin1. The nuclear protein encoded by Lpin1 was named lipin. Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of preadipocytes. These results indicated that lipin is required for normal adipose tissue development and provided a candidate gene for human lipodystrophy. Through database searches, mouse and human EST and genomic sequences with similarities to Lpin1 were identified. These included two related mouse genes (Lpin2 and Lpin3) and three human homologs (LPIN1, LPIN2, and LPIN3). Human LPIN1 gene has been mapped to 2p25.; linkages of fat mass and serum leptin levels to this same region have been noted. Human LPIN2 and LPIN3 mapped to chromosomes 18p11 and 20q11-q12, respectively. The mouse genes encoding Lpin1, Lpin2, and Lpin3 mapped to chromosome 12, 17, and 2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipin 3 Antibody (NB100-53802)(1)

Publication using NB100-53802

• Santos-Rosa H, Leung J, Grimsey N et al. The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J 2005-06-01 [PMID: 15889145]

Bioinformatics

• Gene Symbol: LPIN3
• Entrez:
  • 64900(Human)
• Uniprot:
  • NP_075047.1(Human)