LIN-41/TRIM71 Antibody - BSA Free

Western Blot: LIN-41/TRIM71 Antibody [NBP1-76281] - Human brain tissue lysate with TRIM71/lin-41 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: LIN-41/TRIM71 Antibody - BSA Free [NBP1-76281] - Immunofluorescence of LIN-41/TRIM71 in Human Brain cells with LIN-41/TRIM71 antibody at 20 u/mL.

Immunohistochemistry: LIN-41/TRIM71 Antibody - BSA Free [NBP1-76281] - Immunohistochemistry of LIN-41/TRIM71 in human brain tissue with LIN-41/TRIM71 antibody at 5 u/mL.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LIN-41/TRIM71 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LIN-41/TRIM71 Antibody - BSA Free (NBP1-76281) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71. Amino Acid Squence: CGEKGTKNGQFNYPWD
• Predicted Species: Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM71
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  TRIM71/lin-41 Peptide (NBP1-76281PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LIN-41/TRIM71 Antibody - BSA Free

• LIN41
• LIN-41
• TRIM71
• tripartite motif containing 71
• tripartite motif-containing protein 71

Background

TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development , and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM71
• Uniprot:
  • NP_001034200(Human)