LIM1 Antibody - BSA Free

Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free

Product Discontinued

LIM1 Antibody - BSA Free Summary

• Description: For NB110-12933 specific protocl, please see the PDF under the Product PDFs.
• Immunogen: Synthetic peptide corresponding to C-terminal portion of frog Lim-1.
• Localization: Nuclear
• Specificity: Does not appear to crossreact with LIM5 (the closest homologue of LIM1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LHX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry Whole-Mount 1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1:200
  • Western Blot 1:3000-1:6000
• Application Notes: Immunohistochemistry (Paraffin): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative MEMFA, Immunocytochemistry: 1:500 on P19 cell line. Optimal dilutions to be determined by the researcher.
  
  Do not use tissues fixed overnight in PFA, the antibody will not work.

Reactivity Notes

Frog. Also reacts with Lim-1 from whole-mount fish embryo.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02M PBS, pH 7.6
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Alternate Names for LIM1 Antibody - BSA Free

• hLim-1
• Homeobox protein Lim-1
• LHX1
• LIM homeobox 1
• LIM homeobox protein 1
• LIM1
• LIM-1LIM/homeobox protein Lhx1
• LIM1MGC138141
• MGC126723

Background

The LIM1 gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development o

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIM1 Antibody (NB110-12933)(1)

Publication using NB110-12933

• Bethea M, Liu Y, Wade AK et al. The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis. Am. J. Physiol. Endocrinol. Metab. 2019-01-08 [PMID: 30620636] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: LHX1
• Entrez:
  • 3975(Human)
  • 16869(Mouse)
  • 257634(Rat)
• Uniprot:
  • P29674(Xenopus)