LIM1 Antibody (320416) [Unconjugated]

LIM1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human LIM1 Monoclonal Antibody (Catalog # MAB2725) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 320416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

LIM1 Antibody (320416) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human LIM1
  Leu265-Trp406
  Accession # P48742
• Specificity: Detects human LIM1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LHX1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIM1 Antibody (320416) [Unconjugated]

• hLim-1
• Homeobox protein Lim-1
• LHX1
• LIM homeobox 1
• LIM homeobox protein 1
• LIM1
• LIM-1LIM/homeobox protein Lhx1
• LIM1MGC138141
• MGC126723

Background

LIM homeobox 1 gene product (LIM1), also known as LHX-1, is a homeobox nuclear transcription factor expressed in human brain, thymus, tonsil and many myeloid leukemias. The 406 amino acid human LIM1 contains a LIM domain with twin zinc-binding sequences and a homeobox DNA-binding domain. The C-terminal sequence used as an immunogen is virtually identical between human and mouse LIM1. A truncated human isoform does not include this sequence. LIM1 activity is controlled by interaction with the ubiquitous nuclear adaptor protein Ldb-1 (Lim-domain-binding protein-1) which binds the LIM domain and mediates dimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIM1 Antibody (MAB2725)(5)

Publications using MAB2725

• Hiroaki Kato, Noritaka Saeki, Matome Imai, Hiroshi Onji, Akiko Yano, Shuhei Yoshida et al. LIM1 contributes to the malignant potential of endometrial cancer Frontiers in Oncology 3/10/2023 [PMID: 36969081]
• Hiroaki Kato, Noritaka Saeki, Matome Imai, Hiroshi Onji, Akiko Yano, Shuhei Yoshida et al. LIM1 contributes to the malignant potential of endometrial cancer Frontiers in Oncology 2023-03-10 [PMID: 36969081] (Western Blot, Human, Xenograft)
• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020-04-07;31(1):107471. 2020-04-07 [PMID: 32268087] (ICC, Human)
• Xin Huang, Sophie Balmer, Fan Yang, Miguel Fidalgo, Dan Li, Diana Guallar et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling eLife 11/23/2017 [PMID: 29168693]
• Xin Huang, Sophie Balmer, Fan Yang, Miguel Fidalgo, Dan Li, Diana Guallar et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling eLife 2017-11-23 [PMID: 29168693] (Immunohistochemistry, Mouse)

Bioinformatics

• Gene Symbol: LHX1
• Uniprot:
  • P48742()