LIM kinase 2 [p Thr505] Antibody

Images

 
Western Blot: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Analysis of extracts from Hela cells untreated or treated with PMA using LIM kinase 2 [p Thr505] Antibody.
Immunocytochemistry/ Immunofluorescence: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Staining of methanol-fixed Hela cells using LIM kinase 2 [p Thr505] Antibody.
Immunohistochemistry: LIM kinase 2 [p Thr505] Antibody [NB100-82023] - Analysis of paraffin- embedded human breast carcinoma tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

LIM kinase 2 [p Thr505] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
Modification
p Thr505
Specificity
LIMK2 (specific to Phospho-Thr505) detects endogenous levels of LIMK2 only when phosphorylated at threonine 505.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIMK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LIM kinase 2 [p Thr505] Antibody

  • EC 2.7.11.1
  • LIM domain kinase 2
  • LIMK-2

Background

LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIMK2
Entrez
Uniprot