LIM Kinase 1 Antibody (1A8)

Product Discontinued

LIM Kinase 1 Antibody (1A8) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: LIMK1 (NP_002305 548 a.a. - 647 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD
• Specificity: LIMK1 - LIM domain kinase 1 (1A8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIMK1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. IF usage reported in scientific literature (PMID: 22051374).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LIM Kinase 1 Antibody (1A8)

• EC 2.7.11.1
• LIM domain kinase 1
• LIM Kinase 1
• LIM motif-containing protein kinase
• LIMK
• LIMK1
• LIMK-1
• LIMKLIMK-1

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIM Kinase 1 Antibody (H00003984-M01A)(2)

Publications using H00003984-M01A

• Lu X, Boora U, Seabra L, Rabai EM et al. Knockdown of Slingshot 2 (SSH2) serine phosphatase induces Caspase3 activation in human carcinoma cell lines with the loss of the Birt-Hogg-Dube tumour suppressor gene (FLCN). Oncogene 2013-02-18 [PMID: 23416984] (WB, Human)
• Han L, Wen Z, Lynn RC et al. Regulation of chemotropic guidance of nerve growth cones by microRNA. Molecular Brain34(1):40. 2011-11-01 [PMID: 22051374]

Review for LIM Kinase 1 Antibody (H00003984-M01A) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Maria Rubio WB Human 05/26/2011

Summary

• Application: Western Blot
• Sample Tested: Human brain homogenate
• Species: Human
• Lot: 09133

Bioinformatics

• Gene Symbol: LIMK1
• Entrez:
  • 3984(Human)
• Uniprot:
  • NP_002305(Human)