LGTN Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: LGTN Antibody [NBP1-81308] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & cytosol.

Immunohistochemistry-Paraffin: LGTN Antibody [NBP1-81308] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Western Blot: LGTN Antibody [NBP1-81308] -Analysis in human cell line RPMI-8226.

Immunohistochemistry-Paraffin: LGTN Antibody [NBP1-81308] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: LGTN Antibody [NBP1-81308] - Saining of human colon shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: LGTN Antibody [NBP1-81308] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LGTN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LGTN Antibody - BSA Free (NBP1-81308) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HQAREDKSLSEAPEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRVKKADLPLLTSTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2D
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  LGTN Protein (NBP1-81308PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LGTN Antibody - BSA Free

• eukaryotic translation initiation factor 2D
• HCA56eIF2d
• Hepatocellular carcinoma-associated antigen 56
• LGTNLigatin
• ligatin

Background

LGTN encodes a protein receptor that localizes phosphoglycoproteins within endosomes and at the cell periphery. This trafficking receptor for phosphoglycoproteins may play a role in neuroplasticity by modulating cell-cell interactions, intracellular adhesion, and protein binding at membrane surfaces. In hippocampal neurons, long-lasting down-regulation of ligatin mRNA levels occurs via post-transcriptional RNA processing following glutamate receptor activation. This protein contains single PUA and SUI1 domains and these domains may function in RNA binding and translation initiation, respectively. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2D