Leucyl-cystinyl Aminopeptidase/LNPEP Overexpression Lysate (Native)

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Western Blot: Leucyl-cystinyl aminopeptidase Overexpression Lysate (Adult Normal) [NBL1-12570] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leucyl-cystinyl ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Leucyl-cystinyl Aminopeptidase/LNPEP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC220692 and based on accession number NM_005575. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens leucyl/cystinyl aminopeptidase (LNPEP), transcript variant 1, mRNA.
Gene
LNPEP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
117.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Leucyl-cystinyl Aminopeptidase/LNPEP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Leucyl-cystinyl Aminopeptidase/LNPEP Overexpression Lysate (Native)

  • angiotensin IV receptor
  • AT (4) receptor
  • CAP
  • Cystinyl aminopeptidase
  • EC 3.4.11
  • EC 3.4.11.3
  • Insulin-regulated membrane aminopeptidase
  • Insulin-responsive aminopeptidase
  • IRAP
  • leucyl/cystinyl aminopeptidase
  • Leucylcystinyl Aminopeptidase
  • Leucyl-cystinyl Aminopeptidase
  • LNPEP
  • Otase
  • Oxytocinase
  • Placental leucine aminopeptidase
  • PLAP
  • P-LAP
  • P-LAPinsulin-regulated aminopeptidase
  • Vasopressinase

Background

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Leucyl-cystinyl Aminopeptidase/LNPEP Lysate (NBL1-12570) (0)

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Bioinformatics

Gene Symbol LNPEP
Entrez