Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [Alexa Fluor® 647] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Leucyl-cystinyl Aminopeptidase/LNPEP
  Arg133-Gly552
  Accession # Q9UIQ6
• Specificity: Detects human Leucyl-cystinyl Aminopeptidase/LNPEP in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) ENPEP, rhLRAP, and rhARTS1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leucyl-cystinyl Aminopeptidase/LNPEP Antibody [Alexa Fluor® 647]

• angiotensin IV receptor
• AT (4) receptor
• CAP
• Cystinyl aminopeptidase
• EC 3.4.11
• EC 3.4.11.3
• Insulin-regulated membrane aminopeptidase
• Insulin-responsive aminopeptidase
• IRAP
• leucyl/cystinyl aminopeptidase
• Leucylcystinyl Aminopeptidase
• Leucyl-cystinyl Aminopeptidase
• LNPEP
• Otase
• Oxytocinase
• Placental leucine aminopeptidase
• PLAP
• P-LAP
• P-LAPinsulin-regulated aminopeptidase
• Vasopressinase

Background

LNPEP (also known as P-LAP, CAP, IRAP, and Oxytocinase) is a 165-175 kDa member of the oxytocinase subfamily, M1 family of zinc metallopeptidases. It is widely expressed after cell stimulation and is found on endothelial cells, trophoblasts, skeletal muscle cells, neurons, adipocytes, and renal distal tubule epithelium. LNPEP cleaves vasopressin and oxytocin and serves as a receptor for AngIV. Human LNPEP is a 1025 amino acid (aa) type II transmembrane glycoprotein (SwissProt #:Q9UIQ6). It contains a 110 aa cytoplasmic region (aa 1-110) plus an 894 aa extracellular domain (aa 132-1025) that contains a peptidase M1 region (aa 168‑552). There are multiple potential splice variants. There are two alternative start sites at Met15 and Met20, and the cytoplasmic tail undergoes phosphorylation at Tyr70 and Ser80. LNPEP is reportedly forms homodimers. Proteolytic cleavage between Phe154Ala155 generates a 150-155 kDa soluble form. Over aa 133-552, human LNPEP shares 89% aa sequence identity with mouse LNPEP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.