LEO1 [p Ser10] Antibody

Immunohistochemistry-Paraffin: LEO1 [p Ser10] Antibody [NBP1-72018] - Analysis of anti-LEO1 antibody with human placenta at concentration 5 ug/ml.

Product Discontinued

LEO1 [p Ser10] Antibody Summary

• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human LEO1.
• Modification: p Ser10
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LEO1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for LEO1 [p Ser10] Antibody

• Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• RDLReplicative senescence down-regulated leo1-like protein
• RNA polymerase-associated protein LEO1

Background

LEO1 is a component of the PAF/RNA polymerase II transcription complex. It interacts with parafibromin, PAF1 and CTR9. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LEO1
• Entrez:
  • 123169(Human)
• Uniprot:
  • Q8WVC0(Human)