Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated]

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Legumain/Asparaginyl Endopeptidase Monoclonal Antibody (Catalog # MAB2058) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Legumain/Asparaginyl Endopeptidase at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 301417
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Legumain/Asparaginyl Endopeptidase
  Val18-Tyr435
  Accession # O89017
• Specificity: Detects mouse Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human Legumain is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LGMN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated]

• AEP
• Asparaginyl Endopeptidase
• cysteine protease 1
• Legumain
• LGMN
• LGMN1
• Protease, cysteine 1
• protease, cysteine, 1 (legumain)
• PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP‑2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha ₁ and thymosin alpha ₁₁, two acidic peptides with immunoregulatory properties (5‑7). Mouse Legumain is synthesized as a 435 amino acid precursor with a signal peptide (residues 1 to 17). The pro enzyme (residues 18 to 435) was expressed with an N-terminal His tag. The purified pro enzyme can be activated under the conditions as described above. Legumain activity can be inhibited by rmCystatin C and recombinant human cystatins C and E/M (R&D Systems, Catalog # 1238-PI, 1196-PI, and 1286-PI). 

1. Chen, J.M. et al. (1998) Biochem. J. 335:111.
2. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
3. Liu, C. et al. (2003) Cancer Res. 63: 2957.
4. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
5. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
6. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
7. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Legumain/Asparaginyl Endopeptidase Antibody (MAB2058)(1)

Publication using MAB2058

• Pletneva M, Fan H, Park JJ, Radojcic V, Jie C, Yu Y, Chan C, Redwood A, Pardoll D, Housseau F IFN-producing killer dendritic cells are antigen-presenting cells endowed with T-cell cross-priming capacity. Cancer Res., 2009-08-15;69(16):6607-14. 2009-08-15 [PMID: 19679552] (Western Blot, Mouse)

Review for Legumain/Asparaginyl Endopeptidase Antibody (MAB2058) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 02/16/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22307620
• Species: Mouse

Bioinformatics

• Gene Symbol: LGMN
• Uniprot:
  • O89017()