Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated]

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Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Legumain/Asparaginyl Endopeptidase Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
301417
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Legumain/Asparaginyl Endopeptidase
Val18-Tyr435
Accession # O89017
Specificity
Detects mouse Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human Legumain is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
LGMN
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB2058 in the following application:

Publications
Read Publication using
MAB2058 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody (301417) [Unconjugated]

  • AEP
  • Asparaginyl Endopeptidase
  • cysteine protease 1
  • Legumain
  • LGMN
  • LGMN1
  • Protease, cysteine 1
  • protease, cysteine, 1 (legumain)
  • PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP‑2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (5‑7). Mouse Legumain is synthesized as a 435 amino acid precursor with a signal peptide (residues 1 to 17). The pro enzyme (residues 18 to 435) was expressed with an N-terminal His tag. The purified pro enzyme can be activated under the conditions as described above. Legumain activity can be inhibited by rmCystatin C and recombinant human cystatins C and E/M (R&D Systems, Catalog # 1238-PI, 1196-PI, and 1286-PI). 

  1. Chen, J.M. et al. (1998) Biochem. J. 335:111.
  2. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
  3. Liu, C. et al. (2003) Cancer Res. 63: 2957.
  4. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
  5. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
  6. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
  7. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Legumain/Asparaginyl Endopeptidase Antibody (MAB2058)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Review for Legumain/Asparaginyl Endopeptidase Antibody (MAB2058) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB2058:
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IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22307620
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22307620

Details

Detection NotesSee PMID 22307620

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22307620

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Recent Reviews

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Anonymous
02/16/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol LGMN
Uniprot