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LDOC1 Antibody

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Western Blot: LDOC1 Antibody [NBP1-80323] - Host: Rabbit. Target: LDOC1. Positive control (+): MCF7 (N10). Negative control (-): HeLa (HL). Antibody concentration: 3ug/ml
Western Blot: LDOC1 Antibody [NBP1-80323] - Host: Rabbit. Target: LDOC1. Positive control (+): MCF7 (N10). Negative control (-): HeLa (HL). Antibody concentration: 3ug/ml
Immunohistochemistry: LDOC1 Antibody [NBP1-80323] - Human Thyroid lysate tissue at an antibody concentration of 5.0 ug/ml using anti-LDOC1 antibody
Western Blot: LDOC1 Antibody [NBP1-80323] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: LDOC1 Antibody [NBP1-80323] - Human brain, cortex tissue at an antibody concentration of 5.0 ug/ml using anti-LDOC1 antibody
Immunohistochemistry: LDOC1 Antibody [NBP1-80323] - Human breast tissue at an antibody concentration of 5.0 ug/ml using anti-LDOC1 antibody

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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LDOC1 Antibody Summary

Immunogen
Synthetic peptide directed towards the N terminal of human LDOC1. Peptide sequence MVDELVLLLHALLMRHRALSIENSQLMEQLRLLVCERASLLRQVRPPSCP. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDOC1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Application Notes
This is a rabbit polyclonal antibody against LDOC1 and was validated on Western blot.
Publications
Read Publications using
NBP1-80323 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for LDOC1 Antibody

  • BCUR1
  • breast cancer, up-regulated 1
  • Leucine zipper protein down-regulated in cancer cells
  • leucine zipper, down-regulated in cancer 1
  • Mar7
  • Mart7
  • protein LDOC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: ERG Antibody [NBP2-60655] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ERG at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: ERG Antibody [NBP2-60655] - Total protein from Human THP-1, Jurkat and K562 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ERG in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: ERG Antibody [NBP2-60655] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney (NBP1-78269) using 1:100 dilution of ERG antibody. The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity in the cells of the glomeruli and the tubular epithelial cells.
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Applications: ICC/IF, IHC, IHC-P, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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(9 Reviews)
(35 Publications)
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Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB

Publications for LDOC1 Antibody (NBP1-80323)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
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(2)
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Showing Publications 1 - 2 of 2.
Publications using NBP1-80323 Applications Species
Salemi M, Barone C, Romano C, Caniglia S. LDOC1 expression in fibroblasts of patients with Down syndrome Open Life Sci 2015 (WB, Human) WB Human
Salemi M, Barone N, La Vignera S et al. Leucine zipper, down regulated in cancer-1 gene expression in prostate cancer. Oncol Lett. Oct 1 2016 [PMID: 27698860] (IHC, Human) IHC Human

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LDOC1
Uniprot