LDB3 Antibody

Western Blot: LDB3 Antibody [NBP2-19343] - A. 30 ug RD whole cell lysate/extract 12% SDS-PAGE LDB3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: LDB3 Antibody [NBP2-19343] - Human lung adenocarcinoma, using LDB3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: LDB3 Antibody [NBP2-19343] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: LDB3 Antibody [NBP2-19343] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LDB3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: LDB3 Antibody [NBP2-19343] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LDB3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LDB3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human LDB3. The exact sequence is proprietary.
• Localization: Cytoplasm; perinuclear region; Cell projection; pseudopodium; cytoskeleton; myofibril; sarcomere; Z-disk
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LDB3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for LDB3 Antibody

• CMD1C
• CYPHER
• KIAA01613
• KIAA0613FLJ35865
• LDB3Z1
• LDB3Z4
• LIM domain binding 3
• LIM domain-binding protein 3
• ORACLE
• PDLIM6
• PDZ and LIM domain 6
• Protein cypher
• ZASPLVNC3
• Z-band alternatively spliced PDZ-motif protein

Background

This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1 and 2) have C-terminal LIM domains. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: LDB3
• Uniprot:
  • O75112(Human)