LAT Antibody (627945) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clone: 627945
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

LAT Antibody (627945) [Alexa Fluor® 750] Summary

• Immunogen: Phosphopeptide containing the human LAT Y161 site
  Accession # O43561
• Specificity: Detects human Phospho-LAT (Y161) in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAT Antibody (627945) [Alexa Fluor® 750]

• 36 kDa phospho-tyrosine adapter protein
• LAT
• LAT1,36 kDa phospho-tyrosine adaptor protein
• linker for activation of T cells
• linker for activation of T-cells family member 1
• p36-38
• pp36

Background

LAT (Linker for Activation of T cells) is a type III transmembrane lipid raft protein that serves as a scaffold for signaling molecules. Isoforms of 262 (36 kDa) and 233, called long and short, respectively, differ by inclusion/exclusion of aa 114‑142; a 269 aa isoform lacks this region, but includes alternate N-terminal sequence. Upon T-cell antigen receptor activation, LAT is multiply phosphorylated by ZAP-70/Syk protein tyrosine kinases, creating docking sites for SH2 domain-containing proteins. Phospholipase C-gamma docks at Y161, which is pY132 in the short form of LAT. Mutation of this site results in a Th2 autoimmune lymphoproliferative disorder in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.