LAP1 Antibody (RL13)

Images

 
Immunocytochemistry/ Immunofluorescence: LAP1 Antibody (RL13) [NB120-2737] - Analysis of LAP1 using anti-LAP1 monoclonal antibody shows staining in NS-1 Cells.
Immunohistochemistry-Paraffin: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat lymph node tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% ...read more
Immunohistochemistry: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat breast tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% H202-methanol ...read more
Immunohistochemistry-Paraffin: LAP1 Antibody (RL13) [NB120-2737] - Immunohistochemistry was performed on rat colon tissue. To expose target protein, antigen was retreived. Endogenous peroxidases were blocked in 3% ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
RL13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
13.5 mg/ml

LAP1 Antibody (RL13) Summary

Immunogen
Pore complex-lamina fraction isolated from rat liver nuclear envelopes.
Localization
Immunofluorescence staining of LAP 1 in rat liver with this antibody results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
Specificity
Detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat tissues. This does not cross-react with LAP 2.This detects 3 distinct proteins of molecular weights 75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with NB120-2737 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TOR1AIP1
Purity
PEG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
13.5 mg/ml
Purity
PEG purified

Alternate Names for LAP1 Antibody (RL13)

  • lamina-associated polypeptide 1B
  • torsin A interacting protein 1

Background

In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others.LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only.In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAP1 Antibody (NB120-2737) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB120-2737AF405
Alexa Fluor 488 NB120-2737AF488
Alexa Fluor 647 NB120-2737AF647
Alexa Fluor 700 NB120-2737AF700
DyLight 350 NB120-2737UV
DyLight 405 NB120-2737V
DyLight 488 NB120-2737G
DyLight 680 NB120-2737FR
DyLight 755 NB120-2737IR

Additional LAP1 Products

Array NB120-2737

Bioinformatics Tool for LAP1 Antibody (NB120-2737)

Discover related pathways, diseases and genes to LAP1 Antibody (NB120-2737). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for LAP1 Antibody (NB120-2737)

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Blogs on LAP1

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Bioinformatics

Gene Symbol TOR1AIP1
Entrez
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