Laminin S/Laminin beta 2 Antibody

Product Discontinued

Laminin S/Laminin beta 2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human Laminin beta-2.
• Specificity: Laminin beta 2 (C91) detects endogenous levels of Laminin beta-2 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAMB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Laminin beta 2 (C91) pAb in extracts from RAW264.7 cells.
  Immunohistochemistry (IHC) analyzes of Laminin beta 2 (C91) pAb in human paraffin-embedded lung carcinoma tissue. IF data from customer review.
• Theoretical MW: 210 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Laminin S/Laminin beta 2 Antibody

• LAMB2
• Laminin B1s chain
• Laminin beta 2
• Laminin S
• laminin subunit beta-2
• laminin, beta 2 (laminin S)
• Laminin-11 subunit beta
• Laminin-14 subunit beta
• Laminin-15 subunit beta
• Laminin-3 subunit beta
• Laminin-4 subunit beta
• Laminin-7 subunit beta
• LAMSLaminin-9 subunit beta
• S-LAM beta
• S-laminin subunit beta

Background

The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and gamma. A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The Laminin beta 2 chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin S/Laminin beta 2 Antibody (NBP1-00904)(4)

Publications using NBP1-00904

• Christoffersson G, Walden T, Sandberg M et al. Matrix Metalloproteinase-9 Is Essential for Physiological Beta Cell Function and Islet Vascularization in Adult Mice Am. J. Pathol. 2015-02-07 [PMID: 25665793] (IHC-P, Mouse)
• de Castro Bras Lisandra E, Toba Hiroe, Baicu Catalin F et al. Age and SPARC change the extracellular matrix composition of the left ventricle. Biomed Res Int. 2014-03-24 [PMID: 24783223] (WB, Mouse)
• Van Bockstal M, Lambein K, Gevaert O et al. Stromal architecture and periductal decorin are potential prognostic markers for ipsilateral locoregional recurrence in ductal carcinoma in situ of the breast. Histopathology 2013-05-14 [PMID: 23889174] (IF/IHC, Human)
• Ramirez TA, Jourdan-Le Saux C, Joy A, Zhang J, Dai Q, Mifflin S, Lindsey ML. Chronic and intermittent hypoxia differentially regulate left ventricular inflammatory and extracellular matrix responses. Hypertens Res. 2012-04-12 [PMID: 22495609] (WB, Rat)

Review for Laminin S/Laminin beta 2 Antibody (NBP1-00904) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 06/12/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Human fibroblast cell line
• Species: Human

Bioinformatics

• Gene Symbol: LAMB2
• Entrez:
  • 3913(Human)
  • 25473(Rat)
• Uniprot:
  • P55268(Human)