Human Laminin alpha 3 ELISA Kit (Colorimetric)

ELISA: Human Laminin alpha 3 ELISA Kit (Colorimetric) [NBP3-39748] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 15.625 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 6.1 pg/mL (example only; lot dependent)

Human Laminin alpha 3 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 15.625 - 1000 pg/mL (example only; lot dependent)
• Sensitivity: 6.1 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LAMA3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Laminin alpha 3 ELISA Kit (Colorimetric)

• BM600 150kD subunit
• BM600
• BM600-150kDa
• E170
• Epiligrin 170 kDa subunit
• epiligrin alpha 3 subunit
• Epiligrin subunit alpha
• epiligrin
• kalinin 165kD subunit
• Kalinin subunit alpha
• kalinin-165kDa
• lama3a
• laminin subunit alpha-3
• laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)
• laminin, alpha 3
• laminin-5 alpha 3 chain
• Laminin-5 subunit alpha
• Laminin-6 subunit alpha
• Laminin-7 subunit alpha
• LAMNA
• LOCS
• nicein 150kD subunit
• Nicein subunit alpha
• nicein-150kDa

Background

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Laminin alpha 3 ELISA Kit (NBP3-39748). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LAMA3