Lamin A + C R453W Antibody (12A-2F5)

Images

 
Western Blot: Lamin A + C R453W Antibody (12A-2F5) [NBP1-77401] - Analysis of Lamin A + C R453W in HeLa cells transfected with Flag-tagged Lamin A 1) wild type, 2) R453W and 3) R482W.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
12A-2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Lamin A + C R453W Antibody (12A-2F5) Summary

Immunogen
Synthetic peptide corresponding to amino acids 449-457 of human Lamin A + C [Accession#: NP_733821.1]
Specificity
Disease-associated point-mutant R453W of Lamin A + C
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LMNA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This Lamin A + C R453W (12A-2F5) point-mutant specific antibody is useful for Western blot, ELISA, Immunoprecipitation and Immunocytochemistry/Immunofluorescence. In WB bands can be seen at 74kDa (Lamin A) and 65 kDa (Lamin C).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77401 in the following applications:

Publications
Read Publication using
NBP1-77401 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Lamin A + C R453W Antibody (12A-2F5)

  • 70 kDa Lamin
  • CDCD1
  • CDDC
  • Charcot Marie Tooth Disease Axonal Type 2B1
  • CMD1A
  • CMT2B1
  • EMD2
  • FPL
  • FPLD
  • HGPS
  • IDC
  • LAMIN A
  • Lamin A/C R453W
  • Lamin A/C
  • LAMIN C
  • LDP1
  • LFP
  • LGMD1B
  • LMN 1
  • LMN A
  • LMN C
  • LMN1
  • LMNA
  • LMNC
  • PRELAMIN A
  • PRO1
  • Progeria 1 (Hutchinson Gilford Type)

Background

The lamin A gene is important in making nuclear structure and substructures for DNA replication appropriately in cells. The R453W mutation of LMNA, however, negates much of these normal characteristics, and instead causes the phenotypes of Emery-Dreifuss muscular dystrophy (EDMD). The mutation impairs myoblast formation and differentiation, and contributes to muscle wasting. R453W lamin changes the location of the H2K27me3 modified histone interaction with chromatin, which inactivates certain downstream genes. It also affects the normal lamin function of scaffolding substrates for the MEK-ERK pathway, and disrupts the structure of other binding sites. Thus, R453W seems to cause phenotypes of muscular dystrophy both through direct genetic impact, but also through epigenetic influence over normal scaffolding functionality related to DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lamin A + C R453W Antibody (NBP1-77401)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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IP
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Review for Lamin A + C R453W Antibody (NBP1-77401) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77401:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Lamin A + C R453W NBP1-77401
Enlarge
reviewed by:
Rajeshwari Bhat
WB Human 06/01/2015
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells transfected with lamin A/C R453W
SpeciesHuman
LotA1

Blocking

Blocking Details5% Nonfat dry milk in TBS 0.1% tween-20 at RT for 1hour

Primary Anitbody

Dilution RatiomAb anti Lamin A+C R453W antibody (NBP1-77401) in 5% Nonfat dry milk in TBS 0.1% tween-20 at 4°C O/N. Dilution 1:500

Secondary Antibody

Secondary DescriptionGoat anti-Mouse IgG Secondary Antibody, HRP conjugate Life technologies.
Secondary Manufacturer Cat#Cat No: 32430
Secondary Concentration1:5000

Details

Detection NotesSuperSignal West Dura Substrate used with Molecular Imager VersaDoc MP imaging systems.

Product General Protocols

View specific protocols for Lamin A + C R453W Antibody (NBP1-77401): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lamin A + C R453W Antibody (NBP1-77401) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77401AF405
Alexa Fluor 488 Labeled NBP1-77401AF488
Alexa Fluor 647 Labeled NBP1-77401AF647
Alexa Fluor 700 Labeled NBP1-77401AF700
Biotin Labeled NBP1-77401B
DyLight 350 Labeled NBP1-77401UV
DyLight 405 Labeled NBP1-77401V
DyLight 488 Labeled NBP1-77401G
DyLight 550 Labeled NBP1-77401R
DyLight 650 Labeled NBP1-77401C
DyLight 680 Labeled NBP1-77401FR
DyLight 755 Labeled NBP1-77401IR
FITC Labeled NBP1-77401F
HRP Labeled NBP1-77401H

Additional Lamin A + C R453W Products

Lamin A + C R453W NBP1-77401

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Recent Reviews

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5
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4
1
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0
2
0
1
0

Rajeshwari Bhat
06/01/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LMNA
Entrez
Uniprot