Lamin A + C Antibody (EM-11)

Western Blot: Lamin A + C Antibody (EM-11) [NBP1-50051] - Immunoblots detecting total HA-Dicer (C29F4, HA.11, 3F10) in subcellular fractions of PMEF: HA-Dicer cells. CP and NP fractions were loaded in a 1:3 ratio. HA signals were quantified using ImageJ. CTD S2P, RNA polymerase II carboxy-terminal domain (CTD) phospho-residue serine2; Rad21, cohesin subunit; KDEL motif recognising Grp94/Grp78; H3, histone H3. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007151), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Lamin C Antibody (EM-11) [NBP1-50051] - Staining of lamin C (green) in mouse EL-6 fibroblasts by monoclonal antibody EM-11. DNA stained by DAPI (blue).

Immunocytochemistry/Immunofluorescence: Lamin C Antibody (EM-11) [NBP1-50051] - Staining of lamin C (red) in HeLa cells by monoclonal antibody EM-11. Actin decorated by phalloidin-Alexa Fluor(R) 488 (green), DNA stained by DAPI (blue).

Product Discontinued

Lamin A + C Antibody (EM-11) Summary

• Immunogen: The Lamin C antibody recognizes lamin C an intermediate filament protein of the nuclear lamina.
• Specificity: The mouse monoclonal antibody EM-11 recognizes lamin C, intermediate filament protein of nuclear lamina.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LMNA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1:1000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lamin A + C Antibody (EM-11)

• CDCD1
• CDDC
• CMD1A
• CMT2B1
• dilated 1A (autosomal dominant)
• EMD2
• FPLD
• HGPSFPL
• lamin A/C
• lamin A/C-like 1
• lamin-A/C
• LDP1
• LFP
• LGMD1B
• limb girdle muscular dystrophy 1B (autosomal dominant)
• LMN1IDC
• LMNC
• LMNL1
• prelamin-A/C
• PRO1,70 kDa lamin
• progeria 1 (Hutchinson-Gilford type)
• renal carcinoma antigen NY-REN-32

Background

Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lamin A + C Antibody (NBP1-50051)(3)

Publications using NBP1-50051

• Jin Z, Yang QM, Xiao CY, Gao LL ZP3 and AIPL1 participate in GVBD of mouse oocytes by affecting the nuclear membrane localization and maturation of farnesylated prelamin A Zygote (Cambridge, England) 2022-12-19 [PMID: 36533678]
• Burger K, Gullerova M. Nuclear re-localization of Dicer in primary mouse embryonic fibroblast nuclei following DNA damage. PLoS Genet. 2018-02-01 [PMID: 29394246] (WB, Human)
• Shimi T, Kittisopikul M, Tran J et al. Structural Organization of Nuclear Lamins A, C, B1 and B2 Revealed by Super-Resolution Microscopy. Mol. Biol. Cell 2015-08-26 [PMID: 26310440] (ICC/IF, Mouse)

FAQs for Lamin A + C Antibody (NBP1-50051). (Showing 1 - 1 of 1 FAQs).

1. Would you please help confirm if NBP1-50051 can only recognize Lamin C, not Lamin A?
   • I can confirm that product NBP1-50051 is specific for detection of Lamin C.

Bioinformatics

• Gene Symbol: LMNA