Lamin A + C Antibody (4E7)

Western Blot: Lamin A Antibody (4E7) [NBP2-22180] - Western blot analysis using Lamin A mouse mAb against Raw264.7 (1), PC-12 (2), THP-1 (3), A431 (4), MCF-7 (5) and Jurkat (6) cell lysate.

Immunohistochemistry-Paraffin: Lamin A Antibody (4E7) [NBP2-22180] - Immunohistochemical analysis of paraffin-embedded human striated muscle tissues using Lamin A mouse mAb with DAB staining.

Western Blot: Lamin A Antibody (4E7) [NBP2-22180] - Western blot analysis using Lamin A mAb against human Lamin A (AA: 212-477) recombinant protein. (Expected MW is 56.3 kDa)

ELISA: Lamin A Antibody (4E7) [NBP2-22180] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Discontinued

Lamin A + C Antibody (4E7) Summary

• Immunogen: Purified recombinant fragment of human LMNA expressed in E. Coli.
• Localization: Skeletal muscle: Cytoplasm
• Marker: Nuclear Envelope Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LMNA
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Lamin A + C Antibody (4E7)

• CDCD1
• CDDC
• CMD1A
• CMT2B1
• dilated 1A (autosomal dominant)
• EMD2
• FPLD
• HGPSFPL
• lamin A/C
• lamin A/C-like 1
• lamin-A/C
• LDP1
• LFP
• LGMD1B
• limb girdle muscular dystrophy 1B (autosomal dominant)
• LMN1IDC
• LMNC
• LMNL1
• prelamin-A/C
• PRO1,70 kDa lamin
• progeria 1 (Hutchinson-Gilford type)
• renal carcinoma antigen NY-REN-32

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LMNA