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LAIR2 Antibody Pair [HRP]

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Sandwich ELISA: LAIR2 Antibody Pair [HRP] [NBP2-79308] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Sandwich ELISA: LAIR2 Antibody Pair [HRP] [NBP2-79308] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, S-ELISA
Host
Rabbit
Conjugate
HRP
Standard Curve Range
3.91-250 pg/ml (example only; lot dependent)
Sensitivity
3.9 pg/ml (example only; lot dependent)

Order Details

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Catalog# & Conjugate Size Price
HRP
NBP2-79308-5Plates
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LAIR2 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human LAIR2.
Specificity
This solid phase sandwich ELISA utilizes an antibody specific for Human LAIR2.
Standard Curve Range
3.91-250 pg/ml (example only; lot dependent)
Sensitivity
3.9 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Host
Rabbit
Gene
LAIR2

Applications/Dilutions

Dilutions
  • Immunoprecipitation 5-10 ug/mg lysate
  • Sandwich ELISA
  • Western Blot
Application Notes
1. Capture Antibody (NBP2-89683): Dilute to a working concentration of 2.0 ug/ml in CBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute standard powder with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 250 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 0.08 mg/ml of rabbit anti-LAIR2 monoclonal antibody.
  2. Detection Antibody: 0.5 mg/ml rabbit anti-LAIR2 polyclonal antibody conjugated to horseradish-peroxidase (HRP).
  3. Standard: Each vial contains 17 ng of recombinant LAIR2.

Alternate Names for LAIR2 Antibody Pair [HRP]

  • CD306 antigen
  • CD306
  • CD306leukocyte-associated Ig-like receptor-2
  • LAIR2
  • LAIR-2
  • leukocyte-associated Ig-like receptor 2
  • leukocyte-associated immunoglobulin-like receptor 2
  • MGC71634

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Analysis in human lymph node and pancreas tissues. Corresponding LAIR1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-84590] - Staining of human colon shows moderate to strong membranous positivity in lymphoid cells.
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HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin  alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL).
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
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Western Blot: Chemokine-like factor Antibody [NBP1-59506] - Jurkat cell lysate, concentration 0.2-1 ug/ml.
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AF2940
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Transferase kappa 1 A ...
NBP2-80461
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AF3388
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CCR7 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Small Column Enrichme ...
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Antibody (181703) [Un ...
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Western Blot: CHN 1 Antibody (OTI2G6) [NBP2-46290] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN 1.
Immunocytochemistry/Immunofluorescence: CHN 1 Antibody (OTI2G6) [NBP2-46290] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CHN 1.
Immunohistochemistry-Paraffin: CHN 1 Antibody (OTI2G6) [NBP2-46290] - Analysis of Carcinoma of Human kidney tissue.
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LAIR2 Antibody
Pair [HRP]
NBP2-79308
LAIR2 Antibody Pair [HRP]
Species: Hu
Applications: WB, IP, S-ELISA

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Bioinformatics

Gene Symbol LAIR2