LAIR1 Antibody

Western Blot: LAIR1 Antibody [NBP1-68845] - Analysis of LAIR1 in Human Lymph Node lysate (35ug protein in RIPA buffer) using NBP1-68845 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: LAIR1 Antibody [NBP1-68845] - 3.75ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LAIR1 Antibody Summary

• Description: Novus Biologicals Goat LAIR1 Antibody (NBP1-68845) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NDTEDVSQASPSE corresponding to internal region according to NP_002278.1, NP_068352.1.
• Epitope: C-NDTEDVSQASPSE
• Specificity: This antibody is expected to recognize both isoforms (NP_002278.1; NP_068352.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LAIR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx 28kDa band observed in Human Lymph Node lysates (calculated MW of 29.8kDa according to NP_068352.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Tonsil.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LAIR1 Antibody

• CD305 antigen
• CD305
• CD305leukocyte-associated Ig-like receptor 1
• HLAIR1
• LAIR1
• LAIR-1
• leukocyte-associated immunoglobulin-like receptor 1

Background

CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAIR1
• Entrez:
  • 3903(Human)
• Uniprot:
  • NP_002278.1(Human)