LAIR1 Antibody

Western Blot: LAIR1 Antibody [NBP1-52010] - (2ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LAIR1 Antibody Summary

• Immunogen: internal region
• Epitope: C-NDTEDVSQASPSE
• Specificity: This antibody is expected to recognize both isoforms (NP_002278.1; NP_068352.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LAIR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:1000
  • Western Blot 1-3 ug/ml
• Application Notes: This antibody works in ELISA and Western Blot. Approx 28kDa band observed in Human Lymph Node lysates (calculated MW of 29.8kDa according to NP_068352.1). Recommended concentration: 1-3ug/ml.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LAIR1 Antibody

• CD305 antigen
• CD305
• CD305leukocyte-associated Ig-like receptor 1
• HLAIR1
• LAIR1
• LAIR-1
• leukocyte-associated immunoglobulin-like receptor 1

Background

CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAIR1 Antibody (NBP1-52010)(1)

Publication using NBP1-52010

• Tang X, Narayanan S, Peruzzi G et al. A single residue, arginine 65, is critical for the functional interaction of leukocyte-associated inhibitory receptor-1 with collagens. J Immunol 2009-01-01 [PMID: 19380792]

Bioinformatics

• Gene Symbol: LAIR1
• Entrez:
  • 3903(Human)
• Uniprot:
  • NP_002278.1(Human)