Lactoferricin Antibody (5F12.1.2)

• Reactivity: Bv
• Applications: WB, ELISA
• Clone: 5F12.1.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Lactoferricin Antibody (5F12.1.2) Summary

• Description: Novus Biologicals Mouse Lactoferricin Antibody (5F12.1.2) (NBP3-11195) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Monoclonal antibody clone 5F12.1.2, anti bovine Lactoferricin B is highly specific for bovine Lactoferricin B.
• Epitope: The monoclonal antibody 5F12.1.2 detects the QWR antigenic determinant specific for bovine Lactoferricin B (3kDa).
• Specificity: The monoclonal antibody 5F12.1.2 is specific for bovine Lactoferricin B, it lacks reactivity with bovine lactoferrin C-lobe, human lactoferrin or lactoferricin H. The QWR sequence recognized by the antibody 5F12.1.2 is not present in lactoferrin in human, porcine, mouse, goat, rabbit, equine, rat, cockroach and African clawed frog.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Western Blot
• Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Please notice that the antibody detects a band of 3kDa when western blot is used as application. This asks for a special SDS-Page or Tricine protocol.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Lactoferricin Antibody (5F12.1.2)

• Bovine Lactoferricin
• Lactoferricin B
• LF
• LF-B
• Lfcin B
• Lfcin

Background

Monoclonal antibody clone 5F12.1.2, anti bovine Lactoferricin B is highly specific for bovine Lactoferricin B. This peptide is derived by enzymatic cleavage of lactoferrin which is a member of the transferrin family of metal-binding proteins found in milk and other secretory fluids and also in blood. Cleavage by pepsin of bovine lactoferrin leads to the release of Lactoferricin B (aminoacid 17-41). This peptide is highly basic, possessing five Arg (R) and three Lys (K) residues. In addition, a number of Trp (W) and Phe (F) aromatic residues are present. The two Cys (C) residues from lactoferricin B form a disulfide bond, generating an almost completely cyclical peptide. Nevertheless, the disulfide bond is not required for the antimicrobial potency. Several studies have shown that Lactoferricin B has a broad-spectrum activity against various Gram-positive and Gram-negative bacteria. In addition the peptide has been shown to have antifungal, antiviral and antitumour activity and to bind lipopolysaccharides (LPS, endotoxin). Moreover, it is known to stimulate the adaptive immune response and has anti-inflammatory properties. Lactoferricin B belongs to a large group of cationic antimicrobial peptides. The monoclonal antibody 5F12.1.2 is specific for bovine Lactoferricin B and detects the QWR antigenic determinant specific for bovine Lactoferricin B (3kDa), it lacks reactivity with bovine lactoferrin C-lobe, human lactoferrin or lactoferricin H. The QWR sequence recognized by the antibody 5F12.1.2 is not present in lactoferrin in human, pig, mouse, goat, rabbit, horse, rat, cockroach and African clawed frog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTF