Kynureninase Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kynureninase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4887) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Kynureninase Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynureninase
  Met1-Asn465
  Accession # Q16719
• Specificity: Detects human Kynureninase in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KYNU
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kynureninase Antibody

• EC 3.7.1.3
• KYNU
• kynureninase (L-kynurenine hydrolase)
• Kynureninase
• L-kynurenine hydrolase

Background

Kynureninase is a pyridoxal-5 (-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3-hydroxyanthranilic acid and alanine (1). The enzyme is a member of the “kynurenine pathway” enzymes, through which the majority of dietary tryptophan is degraded in the liver, and is involved in the de novo biosynthesis of NAD⁺ (2, 3). Kynurenine pathway genes are expressed in immune system cells such as macrophages and microglia. During inflammatory responses, the kynurenine pathway in these cells produces quinolinic acid (QA) and not NAD⁺. QA excites neurons via the activation of NMDA (N-methyl-D-aspartate) receptors resulting in neuronal damage. The tissue-damaging process has been demonstrated in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease. Because Kynureninase is one of the key enzymes of QA production, its inhibitors may be useful for the treatment of neurological disorders. The recombinant Kynureninase has been shown to possess specificity for 3-hydroxykynurenine over kynurenine (4, 5).

1. Lima, S. et al. (2007) Biochemistry 46:2735.
2. Botting, N. P. (1995) Chem. Soc. Rev. 24:401.
3. Stone, T. W. (2000) Trends in Pharm. Sci. 21:149.
4. Walsh, H. et al. (2002) Eur. J. Chem. 269:2069.
5. Toma, S. et al. (1997) FEBS Lett. 408:5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kynureninase Antibody (AF4887)(3)

Publications using AF4887

• Lassiter R, Merchen Td, Fang X, Wang Y Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation Frontiers in immunology 7/7/2021 [PMID: 34305900]
• Barton F Haynes HIV-1 Envelope Mimicry of Host Enzyme Kynureninase Does Not Disrupt Tryptophan Metabolism J. Immunol., 2016-11-14;0(0):. 2016-11-14 [PMID: 27849170] (Neutralization)
• Yang , Guang, Holl , T Matt, Liu , Yang, Li , Yi, Lu , Xiaozhi, Nicely , Nathan I, Kepler , Thomas B, Alam , S Munir, Liao , Hua-Xin, Cain , Derek W, Spicer , Leonard, VandeBerg , John L, Haynes , Barton F, Kelsoe , Garnett Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med, 2013-01-28;210(2):241-56. 2013-01-28 [PMID: 23359068] (Western Blot, Human)

Bioinformatics

• Gene Symbol: KYNU
• Uniprot:
  • Q16719()