Kynureninase Antibody (OTI1H1)

Immunohistochemistry-Paraffin: Kynureninase Antibody (1H1) [NBP2-45579] - staining of paraffin-embedded Human lymphoma tissue using anti-KYNU mouse monoclonal antibody. ; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Adenocarcinoma of Human breast tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Carcinoma of Human lung tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Human pancreas tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Carcinoma of Human pancreas tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Human thyroid tissue.

Immunohistochemistry: Kynureninase Antibody (1H1) [NBP2-45579] - Analysis of Human lymph node tissue.

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: OTI1H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Kynureninase Antibody (OTI1H1) Summary

• Description: Novus Biologicals Mouse Kynureninase Antibody (OTI1H1) (NBP2-45579) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-216 of human KYNU (NP_001028170) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KYNU
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
• Theoretical MW: 34.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kynureninase Antibody (OTI1H1)

• EC 3.7.1.3
• KYNU
• kynureninase (L-kynurenine hydrolase)
• Kynureninase
• L-kynurenine hydrolase

Background

KYNU, also known as Kynureninase, has a 465 amino acid long isoform that is 52 kDa and a short 307 amino acid that is 35 kDa; its highest expressed levels are found in placenta, liver and lung; is a PLP dependent enzyme that catalyses the cleavage of kynurenine (Kyn) into anthranilic acid (Ant), and It can also act on 3hKyn (to produce 3hAnt) and some other (3-arylcarbonyl)-alanines, and has cysteine-conjugate-beta-lyase activity. This protein is currently being studied for research on the following diseases and disorders: transient cerebral ischemia, vitamin b deficiency, hydroxykynureninuria, pellagra, cerebritis, ischemia, atopic dermatitis, intrahepatic cholangiocarcinoma, essential hypertension, noma, brain injury, dermatitis, psoriasis cholangiocarcinoma, neurologic diseases, interferon, and neuronitis. Interactions with KYNU protein have been shown to involve 40 proteins including GCK, PIN1, NEDD4L, HSPA9, and ETFA in tryptophan catabolism, metabolism, metabolism of amino acids and derivatives, and NAD metabolism pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KYNU