Kv3.3 Antibody

Western Blot: Kv3.3 Antibody [NB100-58961] - (0.3ug/ml) staining of human brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kv3.3 Antibody Summary

• Immunogen: Peptide with sequence C-KPGPPSFLPDLNAN corresponding to C-Terminus according to NP_004968.2.
• Epitope: KPGPPSFLPDLNAN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 80Da band observed in human brain (frontal cortex) lysates (calculated MW of 80.6 kDa band according to NP_004968.2).
• Control:

  Human Brain Frontal Lobe Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kv3.3 Antibody

• KSHIIID
• KV3.3
• potassium voltage-gated channel subfamily C member 3
• potassium voltage-gated channel, Shaw-related subfamily, member 3
• SCA13
• Shaw-related voltage-gated potassium channel protein 3
• spinocerebellar ataxia 13
• voltage-gated potassium channel protein KV3.3
• Voltage-gated potassium channel subunit Kv3.3

Background

The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kv3.3 Antibody (NB100-58961)(1)

Publication using NB100-58961

• Waters MF, Minassian NA, Stevanin G et al. Mutations in voltage-gated potassium channel KCNC3 cause degenerative and developmental central nervous system phenotypes. Nat Genet 2006-04-01 [PMID: 16501573]

Bioinformatics

• Gene Symbol: KCNC3
• Entrez:
  • 3748(Human)
• Uniprot:
  • NP_004968.2(Human)