Kv4.2 Antibody

Western Blot: Kv4.2 Antibody [NBP3-03748] - Analysis of extracts of various cell lines, using Kv4.2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Kv4.2 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 501-630 of human Kv4.2 (NP_036413.1). ESCMEVATVNRPSSHSPSLSSQQGVTSTCCSRRHKKTFRIPNANVSGSHQGSIQELSTIQIRCVERTPLSNSRSSLNAKMEECVKLNCEQPYVTTAIISIPTPPVTTPEGDDRPESPEYSGGNIVRVSAL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCND2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000
• Application Notes: Kv4.2 antibody validated for IHC-P from a verified customer review.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, pH 7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Kv4.2 Antibody

• potassium voltage-gated channel, Shal-related subfamily, member 2
• RK5
• voltage-gated potassium channel Kv4.2
• voltage-sensitive potassium channel

Background

Kv4.2, a voltage-gated K+ (Kv) channel subunit, has been suggested to be the key component of the subthreshold A-type K+ currents (I(SA)s) recorded from the specific subcellular compartments of certain CNS neurons (1). A-type channels, encoded by the pore-forming alpha-subunits of the Kv4.x family, are particularly important in regulating membrane excitability in the CNS and the heart. Kv4.2 is a Shal-type potassium channel that passes an A-type current and is localized to dendrites and cell bodies in the hippocampus (2). Deletion of the Kv4.2 gene and a loss of Kv4.2 protein resulted in a specific and near-complete elimination of A-type K+ currents from the apical dendrites of CA1 pyramidal neurons. The absence of dendritic Kv4.2-encoded A-type K+ currents led to an increase of bAP amplitude and an increase of concurrent Ca2+ influx (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Kv4.2 Antibody (NBP3-03748) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IHC-P Rat 12/16/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: cardiac tissue
• Species: Rat
• Lot: 0013870201

Comments

• Comments: 1:250 dilution
  Nuclear counterstain

Bioinformatics

• Gene Symbol: KCND2