Ku80/XRCC5 Antibody (SC06-14)

Western Blot: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Western blot analysis of Ku80/XRCC5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Staining Ku80 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ku80/XRCC5 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Staining Ku80 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Staining Ku80 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Ku80 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Analysis of paraffin-embedded human colon cancer tissue using anti-Ku80 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Analysis of paraffin-embedded human kideny tissue using anti-Ku80 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (SC06-14) [NBP2-67583] - Analysis of paraffin-embedded human tonsil tissue using anti-Ku80 antibody. Counter stained with hematoxylin.

Product Discontinued

Ku80/XRCC5 Antibody (SC06-14) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Ku80/XRCC5 aa 693-732 / 732. (SwissProt: P13010 Human)
• Localization: Nucleus, Chromosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: XRCC5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ku80/XRCC5 Antibody (SC06-14)

• 80kDa
• ATP-dependent DNA helicase 2 subunit 2
• ATP-dependent DNA helicase II 80 kDa subunit
• CTC85
• CTCBF
• EC 3.6.4.-
• FLJ39089
• G22P2
• KARP1
• KARP-1
• Ku80
• Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
• Ku86
• Ku86DNA repair protein XRCC5
• KUB2
• NFIV
• Thyroid-lupus autoantigen
• TLAA
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
• X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
• X-ray repair cross-complementing protein 5
• X-ray repair, complementing defective, repair in Chinese hamster
• XRCC5

Background

Ku80, also known as XRCC5, plays a role in many diverse processes including cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis.

As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XRCC5