Ku70/XRCC6 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Ku70/XRCC6 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Ku70
  Asn405-Lys556
  Accession # P12956
• Specificity: Detects endogenous human Ku70 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ku70/XRCC6 Antibody [Alexa Fluor® 350]

• 5'-deoxyribose-5-phosphate lyase Ku70
• 5'-dRP lyase Ku70
• 70 kDa subunit of Ku antigen
• ATP-dependent DNA helicase 2 subunit 1
• ATP-dependent DNA helicase II 70 kDa subunit
• CTC box binding factor 75 kDa subunit
• CTC box-binding factor 75 kDa subunit
• CTC75
• CTCBF
• D22S731
• EC 3.6.4.-
• EC 4.2.99.-
• G22P1
• G22P1Ku70
• Ku autoantigen p70 subunit
• Ku autoantigen, 70kDa
• Ku70
• ML8
• ML8,70 kDa subunit
• thyroid-lupus autoantigen p70
• Thyroid-lupus autoantigen
• TLAA
• TLAAD22S671
• X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6
• X-ray repair cross-complementing protein 6
• XRCC6

Background

Ku70 (Lupus Ku autoantigen protein 70; also ATP-dependent DNA helicase 2 subunit 1) is a ubiquitous 70‑75 kDa nuclear protein that serves as a component of the DNA-dependent protein kinase complex DNA-PK. In particular, it heterodimerizes with Ku80 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku70 is 609 amino acids (aa) in length. It contains a Ku core domain (aa 266‑529), a DNA binding motif (aa 268‑274), an NLS (aa 539‑556) and a C-terminal SAP domain (aa 573‑607). There is one potential splice form that shows a deletion of aa 66‑106. Over aa 405‑556, Ku70 shares 83% aa identity with mouse Ku70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.