Ku70/XRCC6 Antibody (N3H10)

Product Discontinued

Ku70/XRCC6 Antibody (N3H10) Summary

• Immunogen: Human placental extract designated as PSE1-PL
• Epitope: Amino acids 506 - 541.
• Localization: Nuclear
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: XRCC6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Immunoprecipitation 2 ug/ml
  • Knockdown Validated
  • Western Blot 0.25 - 0.5 ug/mL
• Application Notes: May be useful in IHC-P. Flow usage was reported in scientific literature. Knockdown validation (PMID: 31812125).
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Clawed frog. Rat reactivity reported in scientific literature (PMID: 17631504). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Ku70/XRCC6 Antibody (N3H10)

• 5'-deoxyribose-5-phosphate lyase Ku70
• 5'-dRP lyase Ku70
• 70 kDa subunit of Ku antigen
• ATP-dependent DNA helicase 2 subunit 1
• ATP-dependent DNA helicase II 70 kDa subunit
• CTC box binding factor 75 kDa subunit
• CTC box-binding factor 75 kDa subunit
• CTC75
• CTCBF
• D22S731
• EC 3.6.4.-
• EC 4.2.99.-
• G22P1
• G22P1Ku70
• Ku autoantigen p70 subunit
• Ku autoantigen, 70kDa
• Ku70
• ML8
• ML8,70 kDa subunit
• thyroid-lupus autoantigen p70
• Thyroid-lupus autoantigen
• TLAA
• TLAAD22S671
• X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6
• X-ray repair cross-complementing protein 6
• XRCC6

Background

The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ku70/XRCC6 Antibody (NB100-1915)(6)

Publications using NB100-1915

• Bui TM, Butin-Israeli V, Wiesolek HL et al. Neutrophils Alter DNA Repair Landscape to Impact Survival and Shape Distinct Therapeutic Phenotypes of Colorectal Cancer Gastroenterology 2021-03-19 [PMID: 33753103] (Mouse)
• Ji Z, Zhou Y, Wang H et al. Inhibition of DNA Repair Protein Ku70 in High-Glucose Environment Aggravates the Neurotoxicity Induced by Bupivacaine in SH-SY5Y Cells BioMed Research International 2020-01-31 [PMID: 32076604] (WB, Human)
• Loshchenova P, Sergeeva S, Limonov D et al. s Sp1-independent downregulation of NHEJ in response to BER deficiency DNA Repair 2019-11-01 [PMID: 31812125] (KD, WB, Human)
• Wang Y, Lai L, Guo W et al. Inhibition of Ku70 in a high- glucose environment aggravates bupivacaine-induced dorsal root ganglion neurotoxicity Toxicol. Lett. 2019-10-28 [PMID: 31672611] (WB, Mouse)
• Yoon S, Stadler G, Beermann ML et al. Immortalized myogenic cells from congenital muscular dystrophy type1A patients recapitulate aberrant caspase activation in pathogenesis: a new tool for MDC1A research. Skelet Muscle 2013-12-06 [PMID: 24314268] (ICC/IF, Human)
• Doster A, Ziegler S, Foermer S et al. PTO-modified CpG oligodeoxynucleotides mimic autoantigens and reveal a potential role for TLR9 in receptor revision Immunology 2013-01-05 [PMID: 23289794] (FLOW, Human)

Review for Ku70/XRCC6 Antibody (NB100-1915) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ranveer Singh WB Human 06/02/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate from HeLa cells
• Species: Human

Bioinformatics

• Gene Symbol: XRCC6
• Entrez:
  • 2547(Human)
  • 14375(Mouse)
• Uniprot:
  • P12956()