KPNA3 Antibody

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Staining (6ug/ml) of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.

Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NB100-1373] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western Blot: KPNA3 Antibody [NB100-1373] - (0.03ug/ml) staining of CaCo-2 (A) and HEK293 (B), and (0.01ug/ml) MCF7 (C), cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Flow Cytometry: KPNA3 Antibody [NB100-1373] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KPNA3 Antibody Summary

• Description: Novus Biologicals Goat KPNA3 Antibody (NB100-1373) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DPTANLQTKEFNF corresponding to C-Terminus according to NP_002258.2.
• Localization: Cytoplasmic and Nuclear
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KPNA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6 ug/ml
  • Peptide ELISA detection limit 1:128000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: WB: Approx. 60kDa band observed in lysates of cell lines CaCo-2, HEK293 and MCF7 (calculated MW of 57.8kDa according to NP_002258.2). Primary incubation 1 hour at room temperature.
• Control:

  KPNA3 Overexpression Lysate
  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KPNA3 Antibody

• hSRP1
• importin alpha 4
• Importin alpha Q2
• importin alpha-3
• importin subunit alpha-3
• importin-alpha-Q2
• IPOA4
• karyopherin alpha 3 (importin alpha 4)
• Karyopherin subunit alpha-3
• qip2
• SRP1
• SRP1gamma
• SRP1-gamma
• SRP4

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KPNA3
• Entrez:
  • 3839(Human)
  • 16648(Mouse)
• Uniprot:
  • NP_002258.(Human)