KMT2B Antibody

Western Blot: KMT2B Antibody [NBP3-18667] - Figure 2. Western blot analysis using the antibody directed against KMT2B Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse KMT2B , diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of KMT2B is indicated on the right (270 kDa). Cells homozygous for the targeted conditional mll2 allele (delta KMT2B) show a dramatic reduction of KMT2B protein after partial recombination, whereas KMT2B protein was detected in deltaMll1, deltaMll4 and WT E14Tg2a cells.

ELISA: KMT2B Antibody [NBP3-18667] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse KMT2B . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:32,500.

• Reactivity: Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

KMT2B Antibody Summary

• Description: Novus Biologicals Rabbit KMT2B Antibody (NBP3-18667) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against mouse KMT2B (Myeloid/lymphoid or mixed-lineage leukemia protein 2), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal and one peptide containing a sequence from the central part of the protein, respectively.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KMT2B
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:200-1:2000
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for KMT2B Antibody

• EC 2.1.1.43
• histone-lysine N-methyltransferase MLL4
• HRX2
• KIAA0304WW domain-binding protein 7
• Lysine N-methyltransferase 2D
• mixed lineage leukemia gene homolog 2
• MLL2
• myeloid/lymphoid or mixed-lineage leukemia 4
• Myeloid/lymphoid or mixed-lineage leukemia protein 4
• Trithorax homolog 2
• trithorax homologue 2
• TRX2
• WBP7
• WBP-7
• WW domain binding protein 7

Background

MLL4 encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KMT2B
• Uniprot:
  • Q9UMN6(Human)