Glutaredoxin 1/GLRX1 Antibody

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glutaredoxin 1/GLRX1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Glutaredoxin 1
  Met1-Leu107
  Accession # Q9QUH0
• Specificity: Detects endogenous human and mouse Glutaradoxin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GLRX
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutaredoxin 1/GLRX1 Antibody

• GLRX
• glutaredoxin (thioltransferase)
• Glutaredoxin 1
• glutaredoxin-1
• GRXGRX1
• MGC117407
• Thioltransferase
• thioltransferase-1
• TTase-1

Background

Glutaredoxin 1s or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity. Glutaredoxin 1 (GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxin 1s utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the Glutaredoxin 1 system). The amino acid sequence of Glutaredoxin 1 in mouse is highly homologous with that in other mammals (81‑89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutaredoxin 1/GLRX1 Antibody (AF3119)(6)

Publications using AF3119

• Phuong Tuyen Nguyen, Theresa Wacker, Alistair J. P. Brown, Alessandra da Silva Dantas, Elena Shekhova Understanding the Role of Nitronate Monooxygenases in Virulence of the Human Fungal Pathogen Aspergillus fumigatus Journal of Fungi 7/16/2022 [PMID: 35887491]
• Phuong Tuyen Nguyen, Theresa Wacker, Alistair J. P. Brown, Alessandra da Silva Dantas, Elena Shekhova Understanding the Role of Nitronate Monooxygenases in Virulence of the Human Fungal Pathogen Aspergillus fumigatus Journal of Fungi 2022-07-16 [PMID: 35887491] (Western Blot, Mouse)
• Lalida Rojanathammanee, Sharlene Rakoczy, John Kopchick, Holly M. Brown-Borg Effects of insulin-like growth factor 1 on glutathione S-transferases and thioredoxin in growth hormone receptor knockout mice Age (Dordr) 8/1/2014 [PMID: 25001375]
• Lalida Rojanathammanee, Sharlene Rakoczy, John Kopchick, Holly M. Brown-Borg Effects of insulin-like growth factor 1 on glutathione S-transferases and thioredoxin in growth hormone receptor knockout mice Age (Dordr) 2014-08-01 [PMID: 25001375] (Western Blot, Mouse, Transgenic Mouse)
• Valeria Marzano, Simonetta Santini, Claudia Rossi, Mirco Zucchelli, Annamaria D'Alessandro, Carlo Marchetti et al. Proteomic profiling of ATM kinase proficient and deficient cell lines upon blockage of proteasome activity☆ Journal of Proteomics 8/3/2012 [PMID: 22641158]
• Cozzolino M, Amori I, Pesaresi MG, Ferri A, Nencini M, Carri MT Cysteine 111 affects aggregation and cytotoxicity of mutant Cu,Zn-superoxide dismutase associated with familial amyotrophic lateral sclerosis. J. Biol. Chem., 2007-11-15;283(2):866-74. 2007-11-15 [PMID: 18006498] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GLRX
• Uniprot:
  • Q9QUH0()