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![Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/KMT2B-Antibody-Immunohistochemistry-Paraffin-NB300-864-img0003.jpg "Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/KMT2B-Antibody-Immunohistochemistry-Paraffin-NB300-864-img0002.jpg)
![Western Blot: KMT2B Antibody [NBP3-15609] - analysis of various lysates, using KMT2B antibody at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15609_rabbit-kmt2b-pab-western-blot-13102023101735..jpg "Western Blot: KMT2B Antibody [NBP3-15609] - analysis of various lysates, using KMT2B antibody at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: KMT2B Antibody [NBP2-82270] - WB Suggested Anti-MLL4 Antibody Titration: 5ug/ml. Positive Control: HepG2 cell lysateKMT2B is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells](http://images.novusbio.com/fullsize/KMT2B-Antibody-Western-Blot-NBP2-82270-img0001.jpg "Western Blot: KMT2B Antibody [NBP2-82270] - WB Suggested Anti-MLL4 Antibody Titration: 5ug/ml. Positive Control: HepG2 cell lysateKMT2B is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells")
![Western Blot: KMT2B Antibody [NBP2-82270] - Host: Rabbit. Target Name: MLL4. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KMT2B-Antibody-Western-Blot-NBP2-82270-img0002.jpg)
![Western Blot: KMT2B Antibody [NBP3-18667] - Figure 2. Western blot analysis using the antibody directed against KMT2B Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse KMT2B , diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of KMT2B is indicated on the right (270 kDa). Cells homozygous for the targeted conditional mll2 allele (delta KMT2B) show a dramatic reduction of KMT2B protein after partial recombination, whereas KMT2B protein was detected in deltaMll1, deltaMll4 and WT E14Tg2a cells.](http://images.novusbio.com/fullsize/KMT2B-Antibody-Western-Blot-NBP3-18667-img0001.jpg "Western Blot: KMT2B Antibody [NBP3-18667] - Figure 2. Western blot analysis using the antibody directed against KMT2B Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the antibody against mouse KMT2B , diluted 1:500 in BSA/PBS-Tween. The location of the N-terminal fragment of KMT2B is indicated on the right (270 kDa). Cells homozygous for the targeted conditional mll2 allele (delta KMT2B) show a dramatic reduction of KMT2B protein after partial recombination, whereas KMT2B protein was detected in deltaMll1, deltaMll4 and WT E14Tg2a cells.")
![ELISA: KMT2B Antibody [NBP3-18667] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse KMT2B . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:32,500.](http://images.novusbio.com/fullsize/KMT2B Antibody-ELISA-NBP3-18667-img0002.jpg)
![Sandwich ELISA: KMT2B Antibody (1F6) [H00009757-M01] - Detection limit for recombinant GST tagged MLL4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KMT2B-Antibody-1F6-Sandwich-ELISA-H00009757-M01-img0002.jpg "Sandwich ELISA: KMT2B Antibody (1F6) [H00009757-M01] - Detection limit for recombinant GST tagged MLL4 is approximately 0.1ng/ml as a capture antibody.")
![Sandwich ELISA: KMT2B Antibody (4C10) [H00009757-M02] - Detection limit for recombinant GST tagged MLL4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KMT2B-Antibody-4C10-Sandwich-ELISA-H00009757-M02-img0002.jpg "Sandwich ELISA: KMT2B Antibody (4C10) [H00009757-M02] - Detection limit for recombinant GST tagged MLL4 is approximately 0.03ng/ml as a capture antibody.")