KLRG1 Antibody (1151A) [Alexa Fluor™ Plus 555]

• Clone: 1151A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 555

KLRG1 Antibody (1151A) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects mouse KLRG1 in flow cytometry.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLRG1 Antibody (1151A) [Alexa Fluor™ Plus 555]

• 2F1
• CLEC15A
• CLEC15AMAFA-LIKE
• C-type lectin domain family 15 member A
• C-type lectin domain family 15, member A
• ITIM-containing receptor MAFA-L
• killer cell lectin-like receptor subfamily G member 1
• killer cell lectin-like receptor subfamily G, member 1
• KLRG1
• MAFA
• MAFAL
• MAFA-L
• MAFA-like receptor
• MAFAMAFA-2F1
• mast cell function-associated antigen (ITIM-containing)
• Mast cell function-associated antigen
• MGC13600

Background

KLRG1 (Killer cell Lectin‑like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine‑based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8⁺, CD4⁺, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8⁺ T cells in the elderly and by virus‑specific CD8⁺ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
6. Voehringer, D. et al. (2002) Blood 100:3698.
7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
11. Thimme, R. et al. (2005) J. Virol. 79:12112.
12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
15. Banh, C. et al. (2009) Blood 114:5299.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.