KLHL12/C3IP1 Antibody - BSA Free

Western Blot: KLHL12/C3IP1 Antibody [NB120-14233] - E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-KLHL12 )

Product Discontinued

KLHL12/C3IP1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide: KHAKKEREES LPNLLQYVRM PLLTPRYITD VIDAEPFIRC SLQCRDLVDE AKKFHLRPEL RSQMQGPRTR ARLGANEVLL VVGGFGSQQS PIDVVEKYDP KTQEWSFLPS ITRKRRYVAS VSLHDRIYVI GGYDGRSRLS SVECLDYTAD EDGVWYSVAP MNVRRGLAGA TTLGDMIYVS GGFDGSRRHT SMERYDPNID, corresponding to amino acids 205-404 of Human C3IP1. NCBI Accession number: NP_067646.1
• Specificity: C3IP1
• Predicted Species: Xenopus (92%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Chicken
• Gene: KLHL12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: WB: Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28428367).
• Control:

  KLHL12/C3IP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for KLHL12/C3IP1 Antibody - BSA Free

• C3IP1kelch-like protein 12
• CUL3-interacting protein 1
• DKIR
• FLJ27152
• kelch-like 12 (Drosophila)
• kelch-like protein C3IP1

Background

Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3. Component of an ubiquitin-protein E3 ligase complex which includes at least KLHL12/C3IP1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLHL12/C3IP1 Antibody (NB120-14233)(2)

Publications using NB120-14233

• Gorur A, Yuan L, Kenny SJ et al. COPII-coated membranes function as transport carriers of intracellular procollagen-1 bioRxiv 2017-02-21 [PMID: 28428367] (WB, Human)
• McGourty C A, Akopian D et al. Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor. Cell 2016-06-10 [PMID: 27716508] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: KLHL12
• Entrez:
  • 59349(Human)
  • 240756(Mouse)
  • 266772(Rat)
• Uniprot:
  • Q53G59(Human)
  • Q8BZM0(Mouse)
  • Q8R2H4(Rat)