KLF3 Antibody

Images

 
Western Blot: KLF3 Antibody [NB100-1018] - Staining of Human Heart lysate. Antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: KLF3 Antibody [NB100-1018] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Flow Cytometry: KLF3 Antibody [NB100-1018] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: KLF3 Antibody [NB100-1018] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, Flow, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

KLF3 Antibody Summary

Immunogen
Peptide with sequence LMFDPVPVKQEAMD-C corresponding to N-Terminus according to NP_057615.3.
Localization
Nuclear
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
Application Notes
WB: Approx. 40 kDa band observed in human heart lysates (predicted MW of 40 kDa band according to NP_057615). Please note that the 40 kDa band is sometimes accompanied by a 80-90 kDa band of unknown identity.
Control
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1018.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KLF3 Antibody

  • Basic krueppel-like factor
  • basic Kruppel-like factor
  • BKLFbasic kruppel like factor
  • CACCC-box-binding protein BKLF
  • Krueppel-like factor 3
  • Kruppel-like factor 3 (basic)
  • MGC48279
  • TEF-2

Background

The human homolog is a transcription factor involved in haematopoietic differentiation. It binds to the CACCC box.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF3 Antibody (NB100-1018)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KLF3 Antibody (NB100-1018) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Array NB100-1018

Bioinformatics Tool for KLF3 Antibody (NB100-1018)

Discover related pathways, diseases and genes to KLF3 Antibody (NB100-1018). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Product PDFs

Calculators

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Bioinformatics

Gene Symbol KLF3
Entrez
Uniprot