Kirrel2/NEPH3 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Kirrel2/NEPH3 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Kirrel2/NEPH3
  Ser19-Asp501
  Accession # Q7TSU7
• Specificity: Detects mouse Kirrel2/NEPH3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human Kirrel2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kirrel2/NEPH3 Antibody [Alexa Fluor® 647]

• DKFZp564A1164
• Filtrin
• FILTRINkin of IRRE-like protein 2
• kin of IRRE like 2 (Drosophila)
• Kin of irregular chiasm-like protein 2
• Kirrel2
• MGC15718
• NEPH3
• NEPH3Nephrin-like protein 3
• nephrin-like 3
• nephrin-like gene 1
• NLG1
• NLG1kin of irregular chiasm-like 2

Background

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin superfamily (1, 2). Kirrel2 is one of several proteins expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Mouse Kirrel2 cDNA encodes a 700 amino acid (aa) protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589‑598) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% aa identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35‑37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501‑533), while another encodes only portions of Ig domains 1‑4 (5). Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (6). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet beta cell function (7). In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (8). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (9).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.