Kirrel2/NEPH3 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Kirrel2/NEPH3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kirrel2/NEPH3
  Gly21-Arg503
  Accession # Q6UWL6
• Specificity: Detects human Kirrel2/NEPH3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Kirrel2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KIRREL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kirrel2/NEPH3 Antibody [Unconjugated]

• DKFZp564A1164
• Filtrin
• FILTRINkin of IRRE-like protein 2
• kin of IRRE like 2 (Drosophila)
• Kin of irregular chiasm-like protein 2
• Kirrel2
• MGC15718
• NEPH3
• NEPH3Nephrin-like protein 3
• nephrin-like 3
• nephrin-like gene 1
• NLG1
• NLG1kin of irregular chiasm-like 2

Background

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin (Ig) superfamily (1, 2). Kirrel2 is expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Human Kirrel2 cDNA encodes a 708 amino acid (aa) protein that contains a 19 aa signal sequence, a 491 aa extracellular domain with five C2‑type Ig-like domains, a 21 aa transmembrane sequence and a 177 aa intracellular domain with a nephrin-like podocin binding site (aa 597‑606) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of human Kirrel2 shares 86%, 84%, 89% and 87% aa identity with mouse, rat, equine and bovine Kirrel2, respectively. Alternately spliced human Kirrel2 isoforms of 688, 633 and 583 aa have been described (6). These are, respectively, either missing aa 192‑211, or have a 23 aa alternate C-terminus in place of aa 611‑708, with or without aa 22‑71 at the N-terminus. Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (7). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet  beta cell function (8). In the brain, Kirrel2 is present in olfactory neurons, but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (9). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (10).

1. Sun C. et al. (2003) Genomics 82:130.
2. Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.
3. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
4. Sellin, L. et al. (2003) FASEB J. 17:115.
5. NCBI Protein Accession # Q6UWL6.
6. NCBI Protein Accession # AAH64925, NP_115499, and NP_954648.
7. Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.
8. Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.
9. Serizawa, S. et al. (2006) Cell 127:1057.
10. Minaki, Y. et al. (2005) Neurosci. Res. 52:250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kirrel2/NEPH3 Antibody (AF2564)(1)

Publication using AF2564

• GA Higuera, G Iaffaldano, M Bedar, G Shpak, R Broersen, ST Munshi, C Dupont, J Gribnau, FMS de Vrij, SA Kushner, CI De Zeeuw An expandable embryonic stem cell-derived Purkinje neuron progenitor population that exhibits in vivo maturation in the adult mouse cerebellum Sci Rep, 2017-08-18;7(1):8863. 2017-08-18 [PMID: 28821816] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: KIRREL2
• Uniprot:
  • Q6UWL6()