Kirrel2/NEPH3 Antibody [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 680

Kirrel2/NEPH3 Antibody [Alexa Fluor™ Plus 680] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kirrel2/NEPH3
• Specificity: Detects human Kirrel2/NEPH3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Kirrel2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kirrel2/NEPH3 Antibody [Alexa Fluor™ Plus 680]

• DKFZp564A1164
• Filtrin
• FILTRINkin of IRRE-like protein 2
• kin of IRRE like 2 (Drosophila)
• Kin of irregular chiasm-like protein 2
• Kirrel2
• MGC15718
• NEPH3
• NEPH3Nephrin-like protein 3
• nephrin-like 3
• nephrin-like gene 1
• NLG1
• NLG1kin of irregular chiasm-like 2

Background

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin (Ig) superfamily (1, 2). Kirrel2 is expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Human Kirrel2 cDNA encodes a 708 amino acid (aa) protein that contains a 19 aa signal sequence, a 491 aa extracellular domain with five C2‑type Ig-like domains, a 21 aa transmembrane sequence and a 177 aa intracellular domain with a nephrin-like podocin binding site (aa 597‑606) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of human Kirrel2 shares 86%, 84%, 89% and 87% aa identity with mouse, rat, equine and bovine Kirrel2, respectively. Alternately spliced human Kirrel2 isoforms of 688, 633 and 583 aa have been described (6). These are, respectively, either missing aa 192‑211, or have a 23 aa alternate C-terminus in place of aa 611‑708, with or without aa 22‑71 at the N-terminus. Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (7). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet  beta cell function (8). In the brain, Kirrel2 is present in olfactory neurons, but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (9). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (10).

1. Sun C. et al. (2003) Genomics 82:130.
2. Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.
3. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
4. Sellin, L. et al. (2003) FASEB J. 17:115.
5. NCBI Protein Accession # Q6UWL6.
6. NCBI Protein Accession # AAH64925, NP_115499, and NP_954648.
7. Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.
8. Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.
9. Serizawa, S. et al. (2006) Cell 127:1057.
10. Minaki, Y. et al. (2005) Neurosci. Res. 52:250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.