KIF6 Antibody

Immunohistochemistry-Paraffin: KIF6 Antibody [NBP1-44066] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: KIF6 Antibody [NBP1-44066] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KIF6 Antibody Summary

• Description: Novus Biologicals Goat KIF6 Antibody (NBP1-44066) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-KIF6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DPDQNIHLKNLTLH corresponding to internal region according to NP_659464.3.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx. 140kDa band in Human Heart lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.6kDa according to NP_659464.3. The 140kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Prostate and Kidney. .

Reactivity Notes

Expected from sequence similarity: Mouse

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KIF6 Antibody

• C6orf102
• chromosome 6 open reading frame 102
• dJ1043E3.1
• dJ137F1.4
• dJ188D3.1
• DKFZp451I2418
• kinesin family member 6
• kinesin-like protein KIF6
• MGC33317

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIF6 Antibody (NBP1-44066)(1)

Publication using NBP1-44066

• Shiffman D, Chasman DI, Zee RY et al. A kinesin family member 6 variant is associated with coronary heart disease in the Women's Health Study. J Am Coll Cardiol 2008-01-29 [PMID: 18222354]

Bioinformatics

• Gene Symbol: KIF6
• Entrez:
  • 221458(Human)
  • 319991(Mouse)
• Uniprot:
  • NP_659464.3(Human)